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3.4.24.B20: FtsH protease

This is an abbreviated version!
For detailed information about FtsH protease, go to the full flat file.

Word Map on EC 3.4.24.B20

Reaction

degradative cleavage of proteins =

Synonyms

AAA protease, AtFtsH, AtFtsH2, AtFtsH5, AtFtsH6, ATP-dependent zinc metalloprotease, filamentation temperature sensitive H, filamentation temperature sensitive H protease, FtsH, FtsH (slr0228), FtsH metalloprotease, FtsH protease, FtsH protease slr0228, FtsH1, FtsH10, FtsH11, FtsH12, FtsH2, FtsH2 protease, FtsH3, FtsH4, FtsH5, FtsH6, FtsH7, FtsH8, FtsH9, FTSHA, FtsHB, HflB, M41.005, More, putative cell division protein, RISP-degrading activity, slr0228, VAR1, VAR2, zinc dependent protease, ZmFtsH2A, ZmFtsH2B

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.B20 FtsH protease

Cloned

Cloned on EC 3.4.24.B20 - FtsH protease

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
4 alleles of gene var2, positional cloning, initiation of chromosome walking of the var2 gene on chromosome 2, amino acid sequence determination, mechanism of genetic variation
DNA and amino acid sequence determination and analysis, in vitro translation and import into isolated Pisum sativum chloroplasts, where the enzyme is processed to its mature form and targeted into the thylakoid membrane
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) CodonPlus cells
expressed in Escherichia coli C41(DE3) cells
-
expressed in Escherichia coli strain BL21(DE3)
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expressed in Escherichia coli strain BW25113
expressed in Escherichia coli XL1 Blue cells
-
expressed in Synechocystis sp. strain PCC6803
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expression of amino acids 147-610 of FtsH with K410L-K415A surface mutations, and expression of G404L and K207A mutants
expression of GST-tagged wild-type and mutant enzymes in Escherichia coli ftsh-deficient strain A8296
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expression of wild-type enzyme in Escherichia coli strain AR5771
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functional expression in Escherichia coli as GST-fusion protein
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gene ftsh, expression in Escherichia coli
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genes ftsH-1, ftsH-2, ftsH-3, and ftsH-4, DNA and amino acid sequence determination and analysis, quantitative real time RT-PCR expression anaylsis
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genetic allelic variants of gene var2, i.e. AtFtsH2, DNA and amino acid sequence determination and analysis
mutant enzymes are expressed in Escherichia coli AR5088 cells
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ZmFtsH2A, DNA and amino acid sequence determination and analysis, expression of ZmFtsH2A in Nicotiana tabacum transgenic plants using the Agrobacterium tumefaciens strain LBA4404 freeze-thaw transfection method. The full length gene is cloned into a binary vector pCAMBIA1300-35SMCSnos-als containing a constitutive CaMV35S promoter, a nos terminiter, and a mutational chlorsulfuron-resistance acetolactate synthase gene from Arabidopsis thaliana
ZmFtsH2B, DNA and amino acid sequence determination and analysis, expression of ZmFtsH2B in Nicotiana tabacum transgenic plants using the Agrobacterium tumefaciens strain LBA4404 freeze-thaw transfection method. The full length gene is cloned into a binary vector pCAMBIA1300-35SMCSnos-als containing a constitutive CaMV35S promoter, a nos terminiter, and a mutational chlorsulfuron-resistance acetolactate synthase gene from Arabidopsis thaliana