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3.4.24.B11: ADAMTS1 endopeptidase

This is an abbreviated version!
For detailed information about ADAMTS1 endopeptidase, go to the full flat file.

Word Map on EC 3.4.24.B11

Reaction

proteolytic degradation of proteins =

Synonyms

A Disintegrin And Metalloprotease with ThromboSpondin Repeats 1, a disintegrin and metalloprotease with thrombospondin-like motifs, a disintegrin and metalloproteinase with thrombospondin 1, a disintegrin and metalloproteinase with thrombospondin motifs, a disintegrin and metalloproteinase with thrombospondin motifs 1, a disintegrin and metalloproteinase with thrombospondin motifs-1, a disintegrin and metalloproteinase with thrombospondin repeats 1, a disintegrin-like and metalloproteinase domain with thrombospondin type I motifs-1, ADAM metallopeptidase, ADAM-metallopeptidase with thrombospondin type 1 motifs-1, ADAM-TS 1, ADAM-TS1, ADAMTS-1, ADAMTS1, ADAMTS1 matrix metalloproteinase, angiogenesis inhibitor, M12.222, METH-1, METH1, XADAMTS1, Xenopus ortholog of ADAMTS1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.B11 ADAMTS1 endopeptidase

Purification

Purification on EC 3.4.24.B11 - ADAMTS1 endopeptidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CNBr-activated CL-4B-Sepharose affinity chromatography
Hi-trap heparin-sepharose column chromatography and Nickel-chelating chromatography
Ni-NTA Superflow column chromatography, Ni-NTA resin column chromatography, and Superdex 75 gel filtration
Ni2+-probond and anti-v5 antibody-conjugated affinity column chromatography
partially, recombinant His-tagged enzyme from insect cells, 200fold
recombinant enzyme from cell line 293T
-
recombinant His-tagged enzyme from Escherichia coli strain BL21
recombinant His-tagged wild-type and mutants
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recombinant Myc-His-tagged enzyme from cell line 293T
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