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3.4.24.86: ADAM 17 endopeptidase

This is an abbreviated version!
For detailed information about ADAM 17 endopeptidase, go to the full flat file.

Word Map on EC 3.4.24.86

Reaction

Narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-/-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNFalpha). Similarly cleaves other membrane-anchored, cell-surface proteins to "shed" the extracellular domains =

Synonyms

(TACE/ADAM17/CD156q), (TACE:ADAM17), a disintegrin and metallo protease domain 17, a disintegrin and metalloprotease 17, a disintegrin and metalloprotease-17, a disintegrin and metalloproteinase 17, a disintegrin and metalloproteinase-17, ADAM-17, ADAM17, ADAM17 proteinase, ADAM17/tumor necrosis factor-alpha (TNF-A)converting enzyme, ADAMTS-2, CD156, H-TACE, human TACE B, metalloprotease TACE, metalloprotease-disintegrin tumour necrosis factor alpha convertase, metalloproteinase ADAM17, pro tumor necrosis factor cleavage enzyme, pro-tumor necrosis factor-alpha-processing enzyme, proteinase, pro-tumor necrosis factor (9CI), sheddase, TACE, TACE proteinase, TACE/ADAM17, TNF converting enzyme, TNF-alpha convertase, TNF-alpha converting enzyme, TNF-alpha processing protease, TNFalpha converting enzyme, tumor necrosis factor alpha convertase, tumor necrosis factor alpha converting enzyme, tumor necrosis factor alpha-converting enzyme, tumor necrosis factor-alpha converting enzyme, tumor necrosis factor-alpha-converting enzyme, tumour necrosis factor alpha-converting enzyme, tumour necrosis factor-alpha converting enzyme

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.86 ADAM 17 endopeptidase

Cloned

Cloned on EC 3.4.24.86 - ADAM 17 endopeptidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
bone marrow cultures infected with a retrovirus expressing murine TACE cDNA
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cDNA clones coding for various segments of the TACE cytotail
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cDNA encoding mouse TACE, transfection of COS-7 cells
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cDNA encoding TACE, full-length human TACE, inefficiently expressed in insect cells, baculovirus particles in Sf9 cell line from Spodoptera frugiperda
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cDNA fragment coding for the prodomain of human TACE, expressed and purified from a baculoviral expression system
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cloned with retroviral expression plasmids
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expressed in CHO cells
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expressed in MEF cells
expressed in the M1 cell line
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expressed in Trichoplusia Ni cells (High-5 cells9)
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expressed using the baculovirus expression system
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expression in Escherichia coli
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expression in MCF-7 cell
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human embryonic kidney 293 transfectants overexpressing TACE
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isolation of cDNA clones encoding TACE by PCR
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mouse TACE complete cDNA cloned
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PCR used or quantification of TACE-mRNA in fresh tissue
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recombinant TACE expressed in insect cells
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recombinantly expressed
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recombinantly expressed human TACE in plasmid transfected chinese hamster ovary cells
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reconstitution of TNF shedding in TACE-deficient cells by TACE cDNA transfection
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TACE transfected CHO cells
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the catalytic domain of recombinant human TNF-converting enzyme (residues 215-477 with two mutations S266A and N452Q) and a His-tagged tail is purified from the baculovirus/Hi5 cells expression system using affinity chromatography
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transfection of TACE into TACE deficient cells
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