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3.4.24.74: fragilysin

This is an abbreviated version!
For detailed information about fragilysin, go to the full flat file.

Word Map on EC 3.4.24.74

Reaction

Broad proteolytic specificity, bonds hydrolysed including -Gly-/-Leu-, -Met-/-Leu-, -Phe-/-Leu-, -Cys-/-Leu-, Leu-/-Gly =

Synonyms

B. fragilis enterotoxin, Baceroides fragilis enterotoxin, Bacillus fragilis toxin, Bacteroides fragilis enterotoxin, Bacteroides fragilis enterotoxin-2, Bacteroides fragilis toxin, BFT, BFT-1, BFT-2, BFT-3, BFT2, Enterotoxin, FRA3, fragilysin-2, fragilysin-3, Korea-BFT, More, toxin-2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.74 fragilysin

Purification

Purification on EC 3.4.24.74 - fragilysin

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2000-3000fold purified by sequential ammonium sulfate precipitation, ion-exchange chromatography, hydrophobic chromatography, high resolution ion-exchange chromatography
-
BFT-1 and VFT-2
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native enzyme from culture supernatant
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Ni Sepharose column chromatography and Superdex 200 gel filtration
Ni-agarose affinity column chromatography
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Ni-Sepharose column chromatography
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nickel-affinity column chromatography
purified to homogeneity by urea-based purification method
-