3.4.24.72: fibrolase

This is an abbreviated version!
For detailed information about fibrolase, go to the full flat file.

Reaction

Hydrolysis of -Ala14-/-Leu- in insulin B chain and -Lys413-/-Leu- in Aalpha-chain of fibrinogen =

Synonyms

3-203-fibrolase[3-serine], AfeE, Agkistrodon contortrix contortrix metalloproteinase, ALF, Alfimeprase, brevilysin L6, brevithrombolase, broad bean-fibrinolytic enzyme, CFR15-protease, fibrinolytic enzyme, fibrinolytic protease, Fibrinolytic proteinase, FIIa, neuwiedase, Non-hemorrhagic fibrinolytic metalloproteinase, plasmin, Proteinase, Agkistrodon contortrix contortrix venom metallo-, snake venom fibrinolytic enzyme, starase, UFEIII, VlF, wulfase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.24 Metalloendopeptidases

                3.4.24.72 fibrolase

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Results	in table
12	Activating Compound
6	AA Sequence
13	Application
1	CAS Registry Number
6	Cloned(Commentary)
1	Crystallization (Commentary)
120	Disease/ Diagnostics
5	Expression
2	General Information
1	General Stability
1	IC50 Value
183	Inhibitors
1	Ki Value [mM]
33	KM Value [mM]
6	Localization
55	Metals/Ions
29	Molecular Weight [Da]
39	Natural Substrates/ Products (Substrates)
61	Organism
18	pH Optimum
11	pH Range
12	pH Stability
9	pI Value
3	Posttranslational Modification
1	Protein Variants
26	Purification (Commentary)
1	Reaction
1	Reaction Type
61	Reference
1	Renatured (Commentary)
27	Source Tissue
10	Specific Activity [micromol/min/mg]
4	Storage Stability
214	Substrates and Products (Substrate)
36	Subunits
62	Synonyms
20	Temperature Optimum [°C]
8	Temperature Range [°C]
13	Temperature Stability [°C]
30	Turnover Number [1/s]

pH Stability

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pH Stability on EC 3.4.24.72 - fibrolase

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

4 - 12

Bacillus cereus

-

pre-incubation of fibrinolytic protease for 1 h at pH 4.0-12.0 causes substantial activity loss

733238

5 - 11

Neurospora sitophila

-

the enzyme remains stable for 2 h at pH 5.0-11.0, retaining more than 80% activity

754441

5.5

Agkistrodon contortrix contortrix

-

6 h, 30-35% loss of activity

31471

5.8 - 9.2

Streptomyces sp. DPUA 1576

-

protease and fibrinolytic activities are stable during 6 h, at a pH ranging from 6.8 to 8.4 and 5.8 to 9.2, respectively

752378

6 - 12

2 entries

6.5

Agkistrodon contortrix contortrix

-

optimal pH for stability

31471

6.5 - 10

Agkistrodon contortrix contortrix

-

stable

31481

7

Bacillus amyloliquefaciens

-

after incubation for 12 h, the enzyme retains 82.89% of its activity at pH 7.0, 66.03% at pH 8.0, and 50% at pH 6.0 and 9.0 after 8 h of incubation, respectively

754580

7 - 10

Bacillus subtilis

-

after 60 min incubation the enzyme possesses remarkable stability at pH 7.0-10.0 (98.5-100%). In the acidic pH (5.0-6.0), the enzyme retains 77 and 85% activity, respectively

733596

9

Agkistrodon contortrix contortrix

-

6 h, 45-50% loss of activity

31471

additional information

Agkistrodon contortrix contortrix

-

effect of pH on conformation

31481

6 - 12

Serratia sp. KG-2-1

-

the purified enzyme shows higher stability between pH 6.0-8.0 retaining more than 90% of its activity while it retains 87.22 and 74.23% of its activity at pH 5.0 and 9.0, respectively. With further increase in pH 18% residual activity is observed at pH 12.0

752343

6 - 12

Streptomyces sp. CC5

A0A0A7W604

the enzyme activity remains stable after 60 min at pH 6.0-12.0

753870