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3.4.24.72: fibrolase

This is an abbreviated version!
For detailed information about fibrolase, go to the full flat file.

Word Map on EC 3.4.24.72

Reaction

Hydrolysis of -Ala14-/-Leu- in insulin B chain and -Lys413-/-Leu- in Aalpha-chain of fibrinogen =

Synonyms

3-203-fibrolase[3-serine], AfeE, Agkistrodon contortrix contortrix metalloproteinase, ALF, Alfimeprase, brevilysin L6, brevithrombolase, broad bean-fibrinolytic enzyme, CFR15-protease, fibrinolytic enzyme, fibrinolytic protease, Fibrinolytic proteinase, FIIa, neuwiedase, Non-hemorrhagic fibrinolytic metalloproteinase, plasmin, Proteinase, Agkistrodon contortrix contortrix venom metallo-, snake venom fibrinolytic enzyme, starase, UFEIII, VlF, wulfase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.72 fibrolase

Temperature Stability

Temperature Stability on EC 3.4.24.72 - fibrolase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 30
Serratia sp. KG-2-1
-
the enzyme shows relatively high stability between 10-30°C retaining 94.87, 94.83 and 99.79% of activity at respective temperatures while at higher temperature activity decreases
20 - 70
the enzyme activity remains stable for 60 min at 20-30°C and loses about 50% activity after 60 min at 50°C. After 60 min at 60°C, the enzyme completely loses its activity
20 - 80
-
fibrinolytic protease loses about 40-60% of its activity after 1 h incubation at 20-80°C
22
-
stable for 10 days
23 - 60
-
the enzyme is highly thermostable up to 52°C retaining more than 40% of its initial activity when incubated at 23-60°C for 1 h and 2 h
25 - 75
-
the enzyme is stable during 120 min at 25 and 35°C and shows 98, 100, and 59% residual activity at 25, 35 and 45°C, respectively. The enzyme in the crude extract loses its activity after 60 min between 55 and 75°C
30 - 50
-
the enzyme is quite stable at 30-50°C for 60 min, but at 60°C and above activity decreases drastically
30 - 60
-
the enzyme remains stable between 30 and 60°C when incubated for 40 min
60
-
at 60°C, the activity of the enzyme is completely lost
additional information
-
effect of temperature on conformation