3.4.24.72: fibrolase
This is an abbreviated version!
For detailed information about fibrolase, go to the full flat file.
Word Map on EC 3.4.24.72
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3.4.24.72
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plasmin
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thrombolytic
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copperhead
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fibrinogenolytic
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medicine
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nonhemorrhagic
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neuwiedi
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alteplase
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pharmacology
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drug development
- 3.4.24.72
- plasmin
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thrombolytic
- copperhead
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fibrinogenolytic
- medicine
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nonhemorrhagic
- neuwiedi
- alteplase
- pharmacology
- drug development
Reaction
Hydrolysis of -Ala14-/-Leu- in insulin B chain and -Lys413-/-Leu- in Aalpha-chain of fibrinogen =
Synonyms
3-203-fibrolase[3-serine], AfeE, Agkistrodon contortrix contortrix metalloproteinase, ALF, Alfimeprase, brevilysin L6, brevithrombolase, broad bean-fibrinolytic enzyme, CFR15-protease, fibrinolytic enzyme, fibrinolytic protease, Fibrinolytic proteinase, FIIa, neuwiedase, Non-hemorrhagic fibrinolytic metalloproteinase, plasmin, Proteinase, Agkistrodon contortrix contortrix venom metallo-, snake venom fibrinolytic enzyme, starase, UFEIII, VlF, wulfase
ECTree
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Temperature Stability
Temperature Stability on EC 3.4.24.72 - fibrolase
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10 - 30
Serratia sp. KG-2-1
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the enzyme shows relatively high stability between 10-30°C retaining 94.87, 94.83 and 99.79% of activity at respective temperatures while at higher temperature activity decreases
20 - 70
the enzyme activity remains stable for 60 min at 20-30°C and loses about 50% activity after 60 min at 50°C. After 60 min at 60°C, the enzyme completely loses its activity
20 - 80
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fibrinolytic protease loses about 40-60% of its activity after 1 h incubation at 20-80°C
23 - 60
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the enzyme is highly thermostable up to 52°C retaining more than 40% of its initial activity when incubated at 23-60°C for 1 h and 2 h
25 - 75
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the enzyme is stable during 120 min at 25 and 35°C and shows 98, 100, and 59% residual activity at 25, 35 and 45°C, respectively. The enzyme in the crude extract loses its activity after 60 min between 55 and 75°C
30 - 50
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the enzyme is quite stable at 30-50°C for 60 min, but at 60°C and above activity decreases drastically
30 - 60
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the enzyme remains stable between 30 and 60°C when incubated for 40 min
37
additional information
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effect of temperature on conformation
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about 50% loss of activity after 2 days, complete loss of activity after 10 days
37
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after 12 h of incubation, the enzyme remains stable at 4, 25 and 37°C, maintaining activities of 96.04, 95.88, and 98.26%, respectively, and maintains 58.84% of its activity at 40°C
37
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at 37°C, the enzyme residual activity is 94 and 68% of the initial one after 120 and 150 min of incubation, respectively