3.4.24.72: fibrolase

This is an abbreviated version!
For detailed information about fibrolase, go to the full flat file.

Reaction

Hydrolysis of -Ala14-/-Leu- in insulin B chain and -Lys413-/-Leu- in Aalpha-chain of fibrinogen =

Synonyms

3-203-fibrolase[3-serine], AfeE, Agkistrodon contortrix contortrix metalloproteinase, ALF, Alfimeprase, brevilysin L6, brevithrombolase, broad bean-fibrinolytic enzyme, CFR15-protease, fibrinolytic enzyme, fibrinolytic protease, Fibrinolytic proteinase, FIIa, neuwiedase, Non-hemorrhagic fibrinolytic metalloproteinase, plasmin, Proteinase, Agkistrodon contortrix contortrix venom metallo-, snake venom fibrinolytic enzyme, starase, UFEIII, VlF, wulfase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.24 Metalloendopeptidases

                3.4.24.72 fibrolase

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Results	in table
12	Activating Compound
6	AA Sequence
13	Application
1	CAS Registry Number
6	Cloned(Commentary)
1	Crystallization (Commentary)
120	Disease/ Diagnostics
5	Expression
2	General Information
1	General Stability
1	IC50 Value
183	Inhibitors
1	Ki Value [mM]
33	KM Value [mM]
6	Localization
55	Metals/Ions
29	Molecular Weight [Da]
39	Natural Substrates/ Products (Substrates)
61	Organism
18	pH Optimum
11	pH Range
12	pH Stability
9	pI Value
3	Posttranslational Modification
1	Protein Variants
26	Purification (Commentary)
1	Reaction
1	Reaction Type
61	Reference
1	Renatured (Commentary)
27	Source Tissue
10	Specific Activity [micromol/min/mg]
4	Storage Stability
214	Substrates and Products (Substrate)
36	Subunits
62	Synonyms
20	Temperature Optimum [°C]
8	Temperature Range [°C]
13	Temperature Stability [°C]
30	Turnover Number [1/s]

Cloned

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Cloned on EC 3.4.24.72 - fibrolase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

chimeric Arg-Gly-Asp-fibrolase is expressed in Escherichia coli BL21(DE3) cells

Agkistrodon contortrix contortrix

P28891

720980

co-expression of the active signal-less His-tagged enzyme with chaperone DsbC in Escherichia coli cytoplasm, over 90% of the recombinant enzyme is soluble due to DsbC, while the chaperones FkpA and Skp do not have an effect on fibrolase solubility

Agkistrodon contortrix contortrix

P28891

684034

expressed in Pichia pastoris

Agkistrodon contortrix contortrix

-

721082

expression of the wild-type fibrolase and a truncated enzyme form, alfimeprase

Agkistrodon contortrix contortrix

-

684097

gene alf, high level expression of His-tagged alfimeprase in Pichia pastoris strains of GS115 and KM71 by methanol induction, secretion of the recombinant enzyme to the cell culture medium

Agkistrodon contortrix contortrix

P28891

684027

production of alfimeprase by recombinant DNA technology

Agkistrodon contortrix contortrix

-

697595