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3.4.24.69: bontoxilysin

This is an abbreviated version!
For detailed information about bontoxilysin, go to the full flat file.

Word Map on EC 3.4.24.69

Reaction

Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates =

Synonyms

abobotulinumtoxinA, antigen E, Balc424, BoNT, BoNT A, BoNT B, BoNT E, BoNT F, BoNT F/A, BoNT LC, BoNT LC/A, BoNT serotype A, BoNT serotype E, BoNT serotype F, BoNT-A, BoNT-C, BoNT-D, BoNT-E, BoNT-F, BoNT/A, BoNT/A LC, BoNT/A Lc endopeptidase, BoNT/A-LC, BoNT/A1, BoNT/A2, BoNT/A3, BoNT/A4, BoNT/A5, BoNT/A6, BoNT/A8, BoNT/B, BoNT/B light chain protease, BoNT/B-LC, BoNT/B1, BoNT/B6, BoNT/C, BoNT/C1, BoNT/C1-LC, BoNT/C3, BoNT/CD, BoNT/D, BoNT/E, BoNT/F, BoNT/F proteolytic F toxin, BoNT/F-LC, BoNT/F1, BoNT/F5, BoNT/F6, BoNT/F7, BoNT/F9, BoNT/FA, BoNT/G, BoNT/HA, BoNT/T, BoNT/X, BoNTA, BoNTA endopeptidase, BoNTB, BoNTC, BoNTE, BoNTF, Bontoxilysin C1, botox A, botulinum A neurotoxin light chain, Botulinum neurotoxin, botulinum neurotoxin a, botulinum neurotoxin A light chain, botulinum neurotoxin A protease, botulinum neurotoxin A subtype 1, botulinum neurotoxin A subtype 2, botulinum neurotoxin A subtype 6, botulinum neurotoxin A3, botulinum neurotoxin A4, botulinum neurotoxin A8 subtype, botulinum neurotoxin B, botulinum neurotoxin B protease, botulinum neurotoxin C, botulinum neurotoxin D light chain, botulinum neurotoxin E, botulinum neurotoxin endopeptidase, botulinum neurotoxin F, botulinum neurotoxin serotype A, botulinum neurotoxin serotype A endopeptidase, botulinum neurotoxin serotype A light chain, botulinum neurotoxin serotype A protease, botulinum neurotoxin serotype B, botulinum neurotoxin serotype BA, botulinum neurotoxin serotype C1, botulinum neurotoxin serotype C1 light chain protease, botulinum neurotoxin serotype D, botulinum neurotoxin serotype E, botulinum neurotoxin serotype F, botulinum neurotoxin serotype FA, botulinum neurotoxin serotype G, botulinum neurotoxin serotype H, botulinum neurotoxin subtype A, botulinum neurotoxin subtype A3, botulinum neurotoxin subtype A4, botulinum neurotoxin subtype B6, botulinum neurotoxin subtype F5, botulinum neurotoxin type A, botulinum neurotoxin type A light chain, botulinum neurotoxin type B, botulinum neurotoxin type C, botulinum neurotoxin type D, botulinum neurotoxin type E, botulinum neurotoxin type F, botulinum neurotoxin type F light chain, botulinum neurotoxin type G, botulinum neurotoxin type HA, botulinum neurotoxin X, Botulinum neurotoxin-A, botulinum toxin, botulinum toxin C3, botulinum toxin serotype E, botulinum toxin serotype F, botulinum toxin type A, botulinum toxin type B, botulinum toxin type F, Botulinumtoxin A, BoTxA, C2 toxin, CDC69016, Clostridium botulinum A2 neurotoxin, Clostridium botulinum C2 toxin, Clostridium botulinum neurotoxin, Clostridium botulinum neurotoxin A1, Clostridium botulinum neurotoxin F, Clostridium botulinum neurotoxin serotype A, Clostridium botulinum neurotoxin serotype A light chain, Clostridium botulinum neurotoxin type E, Clostridium botulinum serotype D neurotoxin, CNT endopeptidase, D-4947 L-TC, daxibotulinumtoxinA, HCB, HCE, incobotulinumtoxinA, LC/A, LC/D, LC/F5, LC/HA, LC/X, LCA, LcB, lcc1, LCD, LCE, LCF, LHn/D, More, mosaic toxin, neurotoxin A, NT, onabotulinumtoxinA, serotype D botulinum neurotoxin, subtype A4 neurotoxin, TeNT, Tetanus neurotoxin, type A BoNT, type A botulinum neurotoxin, type A botulinum neurotoxin light chain, type A botulinum neurotoxin protease, type F toxin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.69 bontoxilysin

Purification

Purification on EC 3.4.24.69 - bontoxilysin

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE column chromatography, and 4-aminobenzyl 1-thio-beta-D-galactopyranoside agarose affinity column chromatography
-
ammonium sulfate precipitation, DEAE column chromatography, CM Sepharose column chromatography, and Mono-Q column chromatography
ammonium sulfate precipitation, Ni-NTA agarose column chromatography, and DEAE-Sephadex gel filtration
-
anion exchange column chromatography
P10845, Q58GH1
BoNT HCR/A and HCR/B co-purify with synaptic vesicle protein complexes
-
BoNT serotype D and enzyme large toxin complex, L-TC, from strain 4947, by ammonium sulfate fractionation, cation exchange chromatography, another step of ammonium sulfate fractionation, and gel filtration
-
botulinum neurotoxin E light chain
-
botulinum neurotoxin serotype F light chain
-
BuHP Sepharose column chromatography, Q Sepharose column chromatography, and phenyl Sepharose column chromatography
-
C-terminal quarter of the heavy chain of botulinum neurotoxin type A
P10845
cobalt affinity column chromatography, trypsinization, and QAE Sephadex A-50 anion exchange column chromatography
-
commercial preparations of BoNT/A and BoNT/E further purified
-
extraction of enzymatic activity of botulinum neurotoxins/A1, /A2, and /A3 from Clostridium botulinum strain Loch Maree by a panel of monoclonal anti-BoNT/A antibodies, overview
-
full length Clostridium botulinum neurotoxin type E light chain
-
GST-affinity column chromatography and Superdex S200 gel filtration
-
HisTrap column chromatography and phenyl Sepharose column chromatography
-
HisTrap column chromatography and Superdex S200 gel filtration
HisTrap nickel affinity column chromatography
immobilized metal affinity chromatography or GST Sepharose affinity chromatography followed by Superdex 200 gel filtration
P10845
method is based on filtration and chromatography procedures only, which can easily be scaled up from the laboratory purification to industrial needs
-
native BoNT/A free and in complex. Recombinant His-tagged BoNT/A mutant E224A/E262A from Escherichia coli by nickel affinity chromatography, separation of the light and heavy chains
-
native BoNT/A1 and BoNT/A3, by ultrafiltration, hydrophobic interaction and anion exchange chromatography, followed by hydroxyapatite chromatography and dialysis
P10844, P10845
native BoNT/A1, BoNT/A3, BoNT/B1, and BoNT/B4 from strains ATCC 3502, NCTC 2012, Okra, and Eklund 17B strains, respectively, by ultrafiltration, hydrophobic interaction and anion exchange chromatography, followed by hydroxyapatite chromatography and dialysis
P10844, P10845
native BoNT/A2 toxin complex and BoNT/A2 from Clostridium botulinum A2 strain by ammonium sulfate fractionation, and by two different steps of each anion exchange and cation exchange chromatographies
-
native BONT/B from Clostridium botulinum by ion exchange chromatography
-
native BoNT/B1, and BoNT/B4 by ultrafiltration, hydrophobic interaction and anion exchange chromatography, followed by hydroxyapatite chromatography and dialysis
P10844, P10845
Ni-NTA agarose bead chromatography
Ni-NTA agarose column chromatography
Ni-NTA column chromatography
Ni-NTA column chromatography and Superdex 200 gel filtration
Ni-NTA column chromatography and Superdex S200 gel filtration
-
Ni-NTA column chromatography, MonoQ column chromatography, and Superdex 200 gel filtration
-
Ni2+-charged chelating Sepharose column chromatography and phenyl Sepharose column chromatography
recombinant C-terminally His-tagged wild-type and SeMet-labeled BoNT/OFD05 receptor-binding domain from Escherichia coli strain B843 (DE3) by nickel affinity chromatography, dialysis, and gel filtration
recombinant catalytically inactive BoNT/A1 mutant H223A/E224A/H227A holoprotein from Pichia pastoris by cation exchange chromatography to about 95% homogeneity. The purified ciBoNT/A1 HP is a mixed population of singlechain and nicked dichain
-
recombinant GST-tagged HC-fragments pHCAS, pHCBS, pHCCS, pHCDS, pHCES, pHCFS, and pHCGS, and the full-length BoNT/A, BoNT/B, BoNT/D, BoNT/G, and Strep-tagged pBoNTCS-Thro encoding from Escherichia coli by glutathione and streptavidin affinity chromatography, respectively
-
recombinant GST-tagged wild-type and mutant C2II components from Escherichia coli strain BL21
-
recombinant His-tagged and/or FLAG-tagged heavy chains, BoNT/G HCR, BoNT/A HCR, and BoNT/B HCR, from Escherichia coli by nickel affinity chromatography and gel filtration
recombinant His-tagged BoNT AE and EA chimeras from Escherichia coli strain BL21 by immobilized metal affinity and adsorption chromatography, followed by gel filtration, to homogeneity, near-complete conversion of the SC to a disulfide-linked DC as revealed by the appearance of the HC and LC upon SDS-PAGE in the presence of DTT
recombinant His-tagged BoNT/C1(1-430) from Escherichia coli strain B21(DE3) by nickel affinity chromatography
-
recombinant His-tagged BoNT/C1-LC from Escherichia coli strain M15[pREP4] by nickel affinity chromatography
-
recombinant His-tagged BoNT/E fragment HC1163-1256 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged truncated enzyme, residues 1-424, from Escherichia coli strain BL21(DE3) by metal ion affinity chromatography
-
recombinant His6-tagged BoNT/A heavy chain fragment from Escherichia coli by nickel affinity chromatography
P10845
recombinant His6-tagged light chains of the BoNT subtypes from Escherichia coli by nickel affinity chromatography
-
recombinant light chain
-
recombinantly expressed His6-tagged type B botulinum neurotoxin heavy chain transmembrane and binding domain, isolation from Escherichia coli inclusion body, purification, and refolding in a single step by Ni2+ affinity chromatography
-
scale-up, recombinant heavy chain fragment C of botulinum neurotoxin serotype E GS115
-
serotypes BoNT/A to F
-
serotypes BoNT/A, B, E (and their H-chain and L-chain)
-
serotypes BoNT/A, B, E, C, D, F
-
the light chain is purified by nickel affinity column chromatography