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3.4.24.69: bontoxilysin

This is an abbreviated version!
For detailed information about bontoxilysin, go to the full flat file.

Word Map on EC 3.4.24.69

Reaction

Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates =

Synonyms

abobotulinumtoxinA, antigen E, Balc424, BoNT, BoNT A, BoNT B, BoNT E, BoNT F, BoNT F/A, BoNT LC, BoNT LC/A, BoNT serotype A, BoNT serotype E, BoNT serotype F, BoNT-A, BoNT-C, BoNT-D, BoNT-E, BoNT-F, BoNT/A, BoNT/A LC, BoNT/A Lc endopeptidase, BoNT/A-LC, BoNT/A1, BoNT/A2, BoNT/A3, BoNT/A4, BoNT/A5, BoNT/A6, BoNT/A8, BoNT/B, BoNT/B light chain protease, BoNT/B-LC, BoNT/B1, BoNT/B6, BoNT/C, BoNT/C1, BoNT/C1-LC, BoNT/C3, BoNT/CD, BoNT/D, BoNT/E, BoNT/F, BoNT/F proteolytic F toxin, BoNT/F-LC, BoNT/F1, BoNT/F5, BoNT/F6, BoNT/F7, BoNT/F9, BoNT/FA, BoNT/G, BoNT/HA, BoNT/T, BoNT/X, BoNTA, BoNTA endopeptidase, BoNTB, BoNTC, BoNTE, BoNTF, Bontoxilysin C1, botox A, botulinum A neurotoxin light chain, Botulinum neurotoxin, botulinum neurotoxin a, botulinum neurotoxin A light chain, botulinum neurotoxin A protease, botulinum neurotoxin A subtype 1, botulinum neurotoxin A subtype 2, botulinum neurotoxin A subtype 6, botulinum neurotoxin A3, botulinum neurotoxin A4, botulinum neurotoxin A8 subtype, botulinum neurotoxin B, botulinum neurotoxin B protease, botulinum neurotoxin C, botulinum neurotoxin D light chain, botulinum neurotoxin E, botulinum neurotoxin endopeptidase, botulinum neurotoxin F, botulinum neurotoxin serotype A, botulinum neurotoxin serotype A endopeptidase, botulinum neurotoxin serotype A light chain, botulinum neurotoxin serotype A protease, botulinum neurotoxin serotype B, botulinum neurotoxin serotype BA, botulinum neurotoxin serotype C1, botulinum neurotoxin serotype C1 light chain protease, botulinum neurotoxin serotype D, botulinum neurotoxin serotype E, botulinum neurotoxin serotype F, botulinum neurotoxin serotype FA, botulinum neurotoxin serotype G, botulinum neurotoxin serotype H, botulinum neurotoxin subtype A, botulinum neurotoxin subtype A3, botulinum neurotoxin subtype A4, botulinum neurotoxin subtype B6, botulinum neurotoxin subtype F5, botulinum neurotoxin type A, botulinum neurotoxin type A light chain, botulinum neurotoxin type B, botulinum neurotoxin type C, botulinum neurotoxin type D, botulinum neurotoxin type E, botulinum neurotoxin type F, botulinum neurotoxin type F light chain, botulinum neurotoxin type G, botulinum neurotoxin type HA, botulinum neurotoxin X, Botulinum neurotoxin-A, botulinum toxin, botulinum toxin C3, botulinum toxin serotype E, botulinum toxin serotype F, botulinum toxin type A, botulinum toxin type B, botulinum toxin type F, Botulinumtoxin A, BoTxA, C2 toxin, CDC69016, Clostridium botulinum A2 neurotoxin, Clostridium botulinum C2 toxin, Clostridium botulinum neurotoxin, Clostridium botulinum neurotoxin A1, Clostridium botulinum neurotoxin F, Clostridium botulinum neurotoxin serotype A, Clostridium botulinum neurotoxin serotype A light chain, Clostridium botulinum neurotoxin type E, Clostridium botulinum serotype D neurotoxin, CNT endopeptidase, D-4947 L-TC, daxibotulinumtoxinA, HCB, HCE, incobotulinumtoxinA, LC/A, LC/D, LC/F5, LC/HA, LC/X, LCA, LcB, lcc1, LCD, LCE, LCF, LHn/D, More, mosaic toxin, neurotoxin A, NT, onabotulinumtoxinA, serotype D botulinum neurotoxin, subtype A4 neurotoxin, TeNT, Tetanus neurotoxin, type A BoNT, type A botulinum neurotoxin, type A botulinum neurotoxin light chain, type A botulinum neurotoxin protease, type F toxin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.69 bontoxilysin

Cloned

Cloned on EC 3.4.24.69 - bontoxilysin

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
BoNT/A, DNA and amino acid sequence determination, BoNT/A is produced along with six neurotoxin associated proteins, including hemagglutinin Hn-33, through polycistronic expression of a clustered group of genes to form a complex, BoNT/AC. Expresssion of His-tagged BoNT/A mutant E224A/E262A in Escherichia coli
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bont/F, bont/A, and bont/B genes, DNA and amino acid sequence determination and analysis, comparisons, and phylogenetic analysis
bont/F, bont/A, and bont/B genes, DNA and amino acid sequence determination and analysis, phylogenetic analysis
botulinum neurotoxin complex bont genes from different strains, genotyping and phylogenetic analysis, overview
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botulinum neurotoxin complex bont genes from different strains, genotyping and phylogenetic analysis, overview. Synteny among proteolytic, group I, strains or nonproteolytic, group II, strains but not between the two groups
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botulinum neurotoxin serotype F light chain
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botulinum neurotoxin type B strains type B, Ab, and A(B) contain five different subtypes of gene bont/B. B1 subtype gene bont/B DNA sequence determination and analysis. Genomic localization of the bont/B and /A subtype genes, plasmid carriage is bont/B subtype-related. Plasmid-borne bont/B PCR-RFLP subtype genes among Clostridium botulinum strains, overview
botulinum neurotoxin type B strains type B, Ab, and A(B) contain five different subtypes of gene bont/B. B2 subtype gene bont/B DNA sequence determination and analysis. Genomic localization of the bont/B and /A subtype genes, plasmid carriage is bont/B subtype-related. Plasmid-borne bont/B PCR-RFLP subtype genes among Clostridium botulinum strains, overview
botulinum neurotoxin type B strains type B, Ab, and A(B) contain five different subtypes of gene bont/B. Bivalent subtype gene bont/B DNA sequence determination and analysis. Genomic localization of the bont/B and /A subtype genes, plasmid carriage is bont/B subtype-related. Plasmid-borne bont/B PCR-RFLP subtype genes among Clostridium botulinum strains, overview
botulinum neurotoxin type B strains type B, Ab, and A(B) contain five different subtypes of gene bont/B. Non-proteolytic subtype gene bont/B DNA sequence determination and analysis. Genomic localization of the bont/B and /A subtype genes, plasmid carriage is bont/B subtype-related. Plasmid-borne bont/B PCR-RFLP subtype genes among Clostridium botulinum strains, overview
C-terminal quarter of the heavy chain of botulinum neurotoxin type A, expression in Escherichia coli
P10845
catalytically inactive, mutated fragments, designated LHN, comprise the light chain and translocation domains of each neurotoxin and are devoid of any neuron-binding activity. Using codon-optimized genes, LHN fragments, derived from BoNT serotype A, are expressed in Escherichia coli in high yield as soluble proteins
P10844, P10845
catalytically inactive, mutated fragments, designated LHN, comprise the light chain and translocation domains of each neurotoxin and are devoid of any neuron-binding activity. Using codon-optimized genes, LHN fragments, derived from BoNT serotype B, are expressed in Escherichia coli in high yield as soluble proteins
P10844, P10845
catalytically inactive, mutated fragments, designated LHN, comprise the light chain and translocation domains of each neurotoxin and are devoid of any neuron-binding activity. Using codon-optimized genes, LHN fragments, derived from BoNT serotypes A and B, are expressed in Escherichia coli in high yield as soluble proteins
P10844, P10845
cell bank construction, expression of the C-terminal heavy chain fragment of botulinum neurotoxin serotype E in Pichia pastoris strain GS115, method development and evaluation using the pHILD4 Escherichia coli-Pichia pastoris shuttle plasmid, fermentation up-scaling, modeling, overview
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cloning of the gene encoding the BoNT/A heavy chain as 1275 bp AHc fragment synthesized by PCR, and overexpression as His6-tagged protein in Escherichia coli
P10845
Clostridium botulinum
-
construction of an expression vector containing DNA encoding the enterokinase whose cleavage site positioned between LC and HC is replaced with a sequence encoding a second TEV cleavage site, His10- or GFP-tagged, expression in Escherichia coli, generation of a recombinant baculovirus containing BoNT/A LC peptide
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DNA sequence encoding the receptor binding domain of BoNT/CD (S867-E1280) is codon-optimized for expression in Escherichia coli with a C-terminal His tag and cloned into the expression vector pJexpress411 and expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli BL-21(DE3)-RIL cells
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)-RIL cells
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
-
expressed in Escherichia coli BL21-RIL (DE3) cells
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expressed in Escherichia coli BL21-Star (DE3) cells
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expressed in Escherichia coli M15pREP4 cells
expressed in Escherichia coli NiCo21(DE3) cells
P10845
expressed in Escherichia coli SG13009 cells
-
expressed in Escherichia coli TG1
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expressed in nonsporulating and nontoxigenic Clostridium botulinum expression host strain
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expression in Escherichia coli strains BL21(DE3) and M15 of GST-tagged HC-fragments pHCAS, pHCBS, pHCCS, pHCDS, pHCES, pHCFS, and pHCGS, and the full-length BoNT/A, BoNT/B, BoNT/D, BoNT/G, as well as pBoNTCS-Thro encoding full-length BoNT/C fused C-terminally to a Streptag and containing an Escherichia coli protease sensitive peptide between LC and HC
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expression of BoNT serotype A, B, and C light chains
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expression of C-terminally His-tagged BoNT/C1-LC in Escherichia coli strain M15[pREP4]
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expression of catalytically inactive BoNT/A1 mutant H223A/E224A/H227A holoprotein in Pichia pastoris
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expression of GST-tagged wild-type and mutant C2II components in Escherichia coli strain BL21
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expression of heavy chain fragment C of botulinum neurotoxin serotype E in Pichia pastoris
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expression of His-tagged BoNT AE and EA chimeras in Escherichia coli strain BL21
expression of His-tagged BoNT/E fragment, composed by the 1163-1256 residues of botulinum type E neurotoxin HC gene and termed HC1163-1256, in Escherichia coli strain BL21(DE3)
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expression of His-tagged truncated enzyme, residues 1-424, in Escherichia coli strain BL21(DE3)
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expression of His6-tagged light chains of thr BoNT subtypes in Escherichia coli
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expression of N-terminally GST-tagge BoNT/A peptide fragments
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expression of recombinant His-tagged and/or FLAG-tagged heavy chains, BoNT/G HCR, BoNT/A HCR, and BoNT/B HCR, in Escherichia coli strain BL21(DE3)
expression of Strep-tagged HCB-Syt-II fusion protein
-
expression of tagged heavy chain domain, as EGFP-Hc-N/A or mCherry-Hc-N/A, in Escherichia coli strain BL21(DE3)
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expression of the Clostridium botulinum A2 neurotoxin orfX gene cluster proteins ORFX1, ORFX3, P47, and the middle part of NTNH from Clostridium botulinum A2 strain Kyoto F, and NTNH of Clostridium botulinum A1 strain ATCC 3502 in Escherichia coli strain CA434 and in a Clostridium botulinum expression system involving type A transposon Tn916 mutant strain LNT01, expression analysis of orfX cluster genes in native A2 strain, overview
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expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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full length Clostridium botulinum neurotoxin type E light chain, expression in Escherichia coli
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gene bont/F, subtype F7, DNA and amino acid sequence determination and analysis, phylogenetic analysis
genes bont, 6 subtypes, DNA and amino acid sequence determination and analysis, sequence comparisons. The genes from proteolytic, group I, strains form subtypes F1 through F5, while the genes from nonproteolytic, group II, strains form subtype F6, phylogenetic analysis
genes bont, 6 subtypes, DNA and amino acid sequence determination and analysis. The genes from proteolytic, group I, strains form subtypes F1 through F5, while the genes from nonproteolytic, group II, strains form subtype F6, phylogenetic analysis
heavy and light chain DNA sequence determination, expression of GST-tagged wild-type BoNT/A light chain and of seven peptide fragments of BoNT/A light chain, Lc, L1, L2, L1-1, L1-2, L1-3, L1-4, as well as mutant L1-3 BoNT/A light chain peptide fragment in Escherichia coli strain Bl21(DE3)
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high level overexpression by autoinduction method of botulinum neurotoxin serotype C1, comprising residues 1-430, in Escherichia coli strain BL21(DE3) as His-tagged protein
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His6-tagged recombinant type B botulinum neurotoxin heavy chain transmembrane and binding domain, expression in Escherichia coli BL21
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mouse microarray test to identify genes induced by injection of lysophosphatidic acid in a BoNT/C3-reversible manner
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recombinant expression of the C-terminally His-tagged BoNT/OFD05 receptor-binding domain, wild-type and SeMet-labeled, in Escherichia coli strain B843 (DE3)
recombinant light chain type A BoNT LC expressed in Escherichia coli HB101
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regulated expression of serotype B-LC in yeast leads to cleavage of the chimera and a conditional growth defect
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serotypes BoNT/A
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serotypes BoNT/A (3 fragments encompassing the structural gene)
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the heavy chain domain is expressed in Escherichia coli M15 cells
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the receptor-binding domain of botulinum neurotoxin serotype B is expressed in Escherichia coli BL21(DE3) cells