3.4.24.68: tentoxilysin

This is an abbreviated version!
For detailed information about tentoxilysin, go to the full flat file.

Hydrolysis of -Gln76-/-Phe- bond in synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP) =

TeNT, TeNT-Hc, TeNT-LC protein, Tentoxylysin, tetanospasmin, Tetanus neurotoxin, tetanus toxin, TetX

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Results	in table
5	Activating Compound
8	AA Sequence
9	Application
1	CAS Registry Number
9	Cloned(Commentary)
6	Crystallization (Commentary)
6	General Information
11	Inhibitors
3	Localization
18	Metals/Ions
9	Molecular Weight [Da]
42	Natural Substrates/ Products (Substrates)
46	Organism
1	Oxidation Stability
20	PDB ID
2	pH Optimum
2	Posttranslational Modification
35	Protein Variants
9	Purification (Commentary)
5	Reaction
1	Reaction Type
43	Reference
3	Source Tissue
2	Specific Activity [micromol/min/mg]
4	Storage Stability
62	Substrates and Products (Substrate)
10	Subunits
68	Synonyms
4	Temperature Optimum [°C]
1	Temperature Stability [°C]

Storage Stability on EC 3.4.24.68 - tentoxilysin

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-80°C, in 10 mM HEPES buffer, pH 7.2, 50 mM NaCl, after freezing in liquid N2, stable

37°C, enzyme diluted in water supplemented with 5 mg/ml protease-free bovine serum albumin, 3 days, 90% loss of activity

37°C, enzyme diluted in water supplemented with 5 mg/ml protease-free bovine serum albumin, 35-45 days, complete loss of activity

4°C, both native and recombinant TeNT L-chains show significant decreases after 3-4 days of storage