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3.4.24.68: tentoxilysin

This is an abbreviated version!
For detailed information about tentoxilysin, go to the full flat file.

Word Map on EC 3.4.24.68

Reaction

Hydrolysis of -Gln76-/-Phe- bond in synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP) =

Synonyms

TeNT, TeNT-Hc, TeNT-LC protein, Tentoxylysin, tetanospasmin, Tetanus neurotoxin, tetanus toxin, TetX

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.68 tentoxilysin

Purification

Purification on EC 3.4.24.68 - tentoxilysin

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
His-tagged teTN-LC protein
HisTrap column chromatography and Superdex S200 gel filtration
Ni-NTA agarose column chromatography
-
single-chain, two-chain and L-chain form
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TeNT Hc fragment mutants
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tetanus neurotoxin light chain
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very toxic! Booster injection of tetanus toxoid before starting research with tetanus toxin advisable, human anti-tetanus neurotoxin antibodies available
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wild-type and mutated recombinant Hc-fragments
-