3.4.24.59: mitochondrial intermediate peptidase

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For detailed information about mitochondrial intermediate peptidase, go to the full flat file.

Reaction

release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion =

Synonyms

At1g09300, hMIP, Icp55, MIP, MIP1, MIPEP, mitochondrial intermediate peptidase, OCT1, octapeptidyl aminopeptidase 1, Proteinase, mitochondrial intermediate precursor-processing

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.24 Metalloendopeptidases

                3.4.24.59 mitochondrial intermediate peptidase

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Results	in table
1	Activating Compound
3275	AA Sequence
1	Application
1	CAS Registry Number
9	Cloned(Commentary)
11	Disease/ Diagnostics
5	General Information
33	Inhibitors
15	kcat/KM [mM/s]
3	Ki Value [mM]
15	KM Value [mM]
14	Localization
11	Metals/Ions
10	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
21	Organism
3	pH Optimum
1	pH Range
2	Protein Variants
7	Purification (Commentary)
1	Reaction
1	Reaction Type
21	Reference
11	Source Tissue
2	Specific Activity [micromol/min/mg]
2	Storage Stability
103	Substrates and Products (Substrate)
8	Subunits
26	Synonyms
2	Temperature Optimum [°C]
14	Turnover Number [1/s]

Purification

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Purification on EC 3.4.24.59 - mitochondrial intermediate peptidase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Rattus norvegicus

-

31356, 31357

affinity chromatography using Ni-Sepharose resin and ion exchange chromatography with a Hi-trap resource Q column

Homo sapiens

-

718734

affinity purified

Saccharomyces cerevisiae

-

668830

Ni-Sepharose column chromatography and Resource Q column chromatography

Homo sapiens

-

753580

purified 2250fold to homogeneity

Rattus norvegicus

-

668830

recombinant MIPEP protein is purified from baculovirus-infected Sf9 cells

Homo sapiens

-

719949