3.4.24.55: pitrilysin
This is an abbreviated version!
For detailed information about pitrilysin, go to the full flat file.
Word Map on EC 3.4.24.55
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3.4.24.55
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metalloprotease
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metalloendopeptidase
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presequence
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prep
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metallopeptidase
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zinc-binding
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insulin-degrading
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unstructured
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insulysin
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oligopeptidase
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insulinase
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nardilysin
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hxxeh
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peptidasome
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dibasic
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metalloendoprotease
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endoprotease
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b-chain
- 3.4.24.55
- metalloprotease
- metalloendopeptidase
- presequence
-
prep
- metallopeptidase
-
zinc-binding
-
insulin-degrading
- unstructured
- insulysin
-
oligopeptidase
- insulinase
- nardilysin
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hxxeh
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peptidasome
-
dibasic
- metalloendoprotease
- endoprotease
- b-chain
Reaction
Preferential cleavage of -Tyr16-/- Leu- and -Phe25-/- Tyr-bonds of oxidized insulin B chain. Also acts on other substrates of less than 7 kDa such as insulin and glucagon =
Synonyms
EC 3.4.99.44, Escherichia coli protease III, Pitrilysin, ppBH4, Protease Pi, Proteinase Pi, Proteinase, Escherichia coli metallo-, Pi, PTR
ECTree
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Substrates Products
Substrates Products on EC 3.4.24.55 - pitrilysin
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REACTION DIAGRAM
(7-Methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-(2,4-dinitrophenyl)Lys-Leu-Asp-D-Lys + H2O
?
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-
-
-
?
(7-methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-Lys(2,4-dinitrophenyl)-Leu-Asp-D-Lys + H2O
?
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-
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-
?
amyloid beta-peptide with mutations K16A/K28A + H2O
?
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cleavage site is identical to that of native amyloid beta-peptide
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-
?
beta-amyloid + H2O
?
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18% of the activity with beta-endorphin, major cleavage site His14-Gln15
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-
?
beta-endorphin + H2O
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cleavage at Lys19-Asn20 bond. Residues Leu14, Val15 and Leu17 and region 22-26 are responsible for recognition by enzyme
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-
?
Vasoactive intestinal peptide + H2O
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7.2% of the activity with beta-endorphin, major cleavage site Arg14-Lys15
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-
?
?
Halalkalibacterium halodurans
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cleavage at Phe4-Leu5, Leu5-Arg6, Arg6-Arg7, Arg7-Ile8
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-
?
dynorphin A fragment 1-13 + H2O
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Halalkalibacterium halodurans H4
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cleavage at Phe4-Leu5, Leu5-Arg6, Arg6-Arg7, Arg7-Ile8
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-
?
Insulin + H2O
Hydrolyzed insulin
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degradation in such a way that its receptor binding activity is rapidly lost but its precipitability in trichloroacetic acid is only slightly decreased
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-
?
?
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cleavage sites are Asn3-Gln4, His10-Leu11, Ala14-Leu15, Leu17-Val18, Gly23-Phe24, Phe24-Phe25, Phe25-Tyr26, the enzyme shares one of the only two cleavage sites with pitrilysin and 4 sites with insulin-destroying insulinase (EC 3.4.24.56)
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-
?
Insulin B-chain + H2O
?
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small peptides down to 10 residues in length are cleaved more slowly, intact insulin is cleaved very slowly
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-
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Insulin B-chain + H2O
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17% of the activity with beta-endorphin, major cleavage site Tyr16-Leu17
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-
?
Insulin B-chain + H2O
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Halalkalibacterium halodurans
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cleavage at Phe25-Tyr26, Tyr16-Leu17, His10-Leu11
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-
?
Insulin B-chain + H2O
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Halalkalibacterium halodurans H4
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cleavage at Phe25-Tyr26, Tyr16-Leu17, His10-Leu11
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-
?
?
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larger proteins are accepted as substrates to a low extent
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-
?
additional information
?
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prefers the cleavage of small polypeptides to the cleavage of proteins, inactive against synthetic amino acid derivatives of subtilisin, thermolysin, trypsin and chymotrypsin
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-
?
additional information
?
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the activity of the enzyme is confined to substrates smaller than proteins
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-
?
additional information
?
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Halalkalibacterium halodurans
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no substrate: 4-nitroanilides of several amino acids tested, azocasein, bovine serum albumin
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-
?
additional information
?
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Halalkalibacterium halodurans H4
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no substrate: 4-nitroanilides of several amino acids tested, azocasein, bovine serum albumin
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-
?