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3.4.24.55: pitrilysin

This is an abbreviated version!
For detailed information about pitrilysin, go to the full flat file.

Word Map on EC 3.4.24.55

Reaction

Preferential cleavage of -Tyr16-/- Leu- and -Phe25-/- Tyr-bonds of oxidized insulin B chain. Also acts on other substrates of less than 7 kDa such as insulin and glucagon =

Synonyms

EC 3.4.99.44, Escherichia coli protease III, Pitrilysin, ppBH4, Protease Pi, Proteinase Pi, Proteinase, Escherichia coli metallo-, Pi, PTR

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.55 pitrilysin

Substrates Products

Substrates Products on EC 3.4.24.55 - pitrilysin

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(7-Methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-(2,4-dinitrophenyl)Lys-Leu-Asp-D-Lys + H2O
?
show the reaction diagram
-
-
-
-
?
(7-methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-Lys(2,4-dinitrophenyl)-Leu-Asp-D-Lys + H2O
?
show the reaction diagram
-
-
-
-
?
amyloid beta-peptide + H2O
?
show the reaction diagram
-
-
-
-
?
amyloid beta-peptide with mutations K16A/K28A + H2O
?
show the reaction diagram
-
cleavage site is identical to that of native amyloid beta-peptide
-
-
?
angiotensinogen(1-14) + H2O
?
show the reaction diagram
-
-
-
-
?
beta-amyloid + H2O
?
show the reaction diagram
-
18% of the activity with beta-endorphin, major cleavage site His14-Gln15
-
-
?
beta-endorphin + H2O
?
show the reaction diagram
-
cleavage at Lys19-Asn20 bond. Residues Leu14, Val15 and Leu17 and region 22-26 are responsible for recognition by enzyme
-
-
?
calcitonin + H2O
?
show the reaction diagram
-
3% of the activity with beta-endorphin
-
-
?
dynorphin + H2O
?
show the reaction diagram
dynorphin A fragment 1-13 + H2O
?
show the reaction diagram
Glucagon + H2O
?
show the reaction diagram
Insulin + H2O
Hydrolyzed insulin
show the reaction diagram
Insulin B-chain + H2O
?
show the reaction diagram
Insulin-like growth factor II + H2O
?
show the reaction diagram
-
-
-
-
?
Secretin + H2O
?
show the reaction diagram
-
-
-
-
?
Substance P + H2O
?
show the reaction diagram
-
10% of the activity with beta-endorphin
-
-
?
Thyrocalcitonin + H2O
?
show the reaction diagram
-
-
-
-
?
Vasoactive intestinal peptide + H2O
?
show the reaction diagram
-
7.2% of the activity with beta-endorphin, major cleavage site Arg14-Lys15
-
-
?
additional information
?
-