3.4.24.55: pitrilysin

This is an abbreviated version!
For detailed information about pitrilysin, go to the full flat file.

Reaction

Preferential cleavage of -Tyr16-/- Leu- and -Phe25-/- Tyr-bonds of oxidized insulin B chain. Also acts on other substrates of less than 7 kDa such as insulin and glucagon =

Synonyms

EC 3.4.99.44, Escherichia coli protease III, Pitrilysin, ppBH4, Protease Pi, Proteinase Pi, Proteinase, Escherichia coli metallo-, Pi, PTR

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.24 Metalloendopeptidases

                3.4.24.55 pitrilysin

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
5806	AA Sequence
1	CAS Registry Number
2	Cloned(Commentary)
47	Inhibitors
13	KM Value [mM]
8	Localization
15	Metals/Ions
10	Molecular Weight [Da]
15	Organism
1	PDB ID
3	pH Optimum
1	pI Value
16	Protein Variants
5	Purification (Commentary)
1	Reaction
1	Reaction Type
15	Reference
1	Specific Activity [micromol/min/mg]
1	Storage Stability
43	Substrates and Products (Substrate)
7	Subunits
9	Synonyms
3	Temperature Optimum [°C]
3	Temperature Stability [°C]
10	Turnover Number [1/s]

KM Value

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

KM Value on EC 3.4.24.55 - pitrilysin

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to KM Value Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0077

(7-methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-Lys(2,4-dinitrophenyl)-Leu-Asp-D-Lys

Escherichia coli

-

-

31306

0.0009

beta-amyloid

Escherichia coli

-

pH 8.0, 37°C

668004

-

0.00036

beta-endorphin

Escherichia coli

-

pH 8.0, 37°C

668004

0.0022

calcitonin

Escherichia coli

-

pH 8.0, 37°C

668004

0.12

dynorphin A fragment 1-13

Halalkalibacterium halodurans

-

pH 8.0, 37°C

667795

0.00061 - 0.0127

glucagon

2 entries

0.000105

Insulin

Acinetobacter calcoaceticus

-

-

31309, 31310

-

0.0015 - 0.05

Insulin B-chain

2 entries

0.031

Substance P

Escherichia coli

-

pH 8.0, 37°C

668004

0.0004

Vasoactive intestinal peptide

Escherichia coli

-

pH 8.0, 37°C

668004

0.00061

glucagon

Acinetobacter calcoaceticus

-

-

31310

0.0127

glucagon

Escherichia coli

-

-

31306

0.0015

Insulin B-chain

Escherichia coli

-

pH 8.0, 37°C

668004

0.05

Insulin B-chain

Halalkalibacterium halodurans

-

pH 8.0, 37°C

667795