3.4.24.47: horrilysin
This is an abbreviated version!
For detailed information about horrilysin, go to the full flat file.
Reaction
Cleavage of only the single bond Ala14-/-Leu in the insulin B chain, Ser12-/-Leu in the A chain, and Ile-/-Gly, Pro-/-Ala, and Ser-/-Trp in melittin =
Synonyms
Crotalus horridus metalloendopeptidase, Hemorrhagic proteinase IV, HP-IV, Proteinase, Crotalus horridus horridus venom hemorrhagic
ECTree
3.4.24.47 horrilysinAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.4.24.47 - horrilysin
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Bee venom melittin + H2O
?
-
cleaves at Ile2-Gly3, Pro14-Ala15, Ser18-Trp19
-
-
?
cow hide powder + H2O
?
-
hydrolysis only at 20% of the rate at which hide powder azure is hydrolyzed
-
-
?
Fibrinogen + H2O
?
-
rapid digestion of the Aalpha chain followed by a slower degradation of the Bbeta chain
-
-
?
Fibrinogen + H2O
Hydrolyzed fibrinogen
-
hydrolysis of alpha and beta chain
-
?
glomerular basement membrane + H2O
?
-
catalysis of the complete solubilization
-
-
?
Hide powder + H2O
?
-
from cow, hydrolysis at 20% the rate of cow hide powder azure
-
-
?
insulin + H2O
?
-
cleavage of the Val-Glu-Ala15-Leu16-Tyr-Leu bond of the B chain and Val-Cys-Ser12-Leu13-Tyr-Gly bond of the A chain
-
-
?
Melittin + H2O
?
-
cleavage at the Ile2-Gly3, Pro14-Ala15 and Ser18-Trp19 bonds
-
-
?
Oxidized insulin A-chain + H2O
Hydrolyzed oxidized insulin A-chain
-
cleavage site: Ser12-Leu13
-
?
Oxidized insulin B-chain + H2O
Hydrolyzed oxidized insulin B-chain
-
cleavage site: Ala14-Leu15
-
?
Fibrin + H2O
?
-
thrombin-induced fibrin, hydrolysis of alpha chain
-
-
?
additional information
?
-
-
no substrates are elastin (as elastin-orcein or elastin-congo red)
-
-
?
additional information
?
-
-
no substrates are keratin (as keratin azure)
-
-
?
additional information
?
-
-
no substrates are N-carbobenzoxy-Ser-Leu-NH2, N-carbobenzoxy-Ala-Leu-NH2, N-carbobenzoxy-Ile-Gly-NH2, N,N-dimethylcasein, N,N-dimethylhemoglobin or several dipeptides
-
-
?
additional information
?
-
-
no cleavage of various unblocked dipeptides and the doubly blocked dipeptides Z-Ser-Leu-NH2, Z-Ala-Leu-NH2 and Z-Ile-Gly-NH2
-
-
?
