3.4.24.44: atrolysin E
This is an abbreviated version!
For detailed information about atrolysin E, go to the full flat file.
Word Map on EC 3.4.24.44
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3.4.24.44
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venom
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rattlesnake
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diamondback
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snake
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metalloproteinase
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disintegrins
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zymogen
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spacer
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raman
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proteinases
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medicine
- 3.4.24.44
- venom
- rattlesnake
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diamondback
-
snake
- metalloproteinase
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disintegrins
- zymogen
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spacer
-
raman
- proteinases
- medicine
Reaction
Cleavage of Asn3-/-Gln, Ser9-/-His and Ala14-/-Leu bonds in insulin B chain and Tyr14-/-Gln and Thr8-/-Ser in A chain. Cleaves type IV collagen at Ala73-/-Gln in alpha1(IV) and at Gly7-/-Leu in alpha2(IV) =
Synonyms
atrolysin E/D, Crotalus atrox metalloendopeptidase e, Hemorrhagic toxin e, HT-e
ECTree
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Substrates Products
Substrates Products on EC 3.4.24.44 - atrolysin E
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REACTION DIAGRAM
basement membrane preperation from Engel-breth-Holm-Swarm tumor + H2O
?
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great proteolytic activity
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-
?
Collagen type IV alphaI(IV) chains + H2O
?
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basement membrane component, cleavage at Ala219-Gln220
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-
?
Collagen type IV alphaII(IV) chain + H2O
?
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basement membrane component, cleavage at Thr228-Leu229
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-
?
Fibrinogen + H2O
?
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rapid cleavage of the Aalpha chain followed by the Bbeta and gamma chains
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-
?
Human alpha2-macroglobulin + H2O
?
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cleavage at Val689-Met690 and Gly693-His694
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-
?
Laminin/nidogen complex + H2O
?
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cleavage at positions 322, 336, 351, 840 and 953
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-
?
Oxidized insulin A-chain + H2O
Hydrolyzed oxidized insulin A-chain
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rapid cleavage at Tyr14-Gln15, more slowly at Ala8-Ser9, kinetics
peptides (Gln15-Asn21) + (Gly1-Tyr14) + (Ser9-Tyr14) + (Gly1-Ala8)
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Basement membrane preparation + H2O
Soluble peptides
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component band a, most active on this substrate
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?
?
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contributes to hemorrhagic property by weakening capillary walls, together with atrolysins B and C member of the class P-I hemorrhagic toxins
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-
?
Basement membranes surrounding capillaries + H2O
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together with hemorrhagic toxins a, b, c, d and f responsible for hemorrhage
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-
?
Laminin + H2O
?
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basement membrane component, most active on this substrate
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-
?
Nidogen + H2O
?
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basement membrane component, cleavage at positions 75, 296, 336, 402, 478, 625, 702 and 920
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-
?
Hydrolyzed oxidized insulin B-chain
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-
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?
Oxidized insulin B-chain + H2O
Hydrolyzed oxidized insulin B-chain
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cleavage sites: Ser9-His10, Ala14-Leu15 (very rapidly)
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-
?
Oxidized insulin B-chain + H2O
Hydrolyzed oxidized insulin B-chain
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kinetics
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-
?
Oxidized insulin B-chain + H2O
Hydrolyzed oxidized insulin B-chain
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cleavage sites: Asn3-Gln4 (very slowly)
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-
?
additional information
?
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no hydrolysis of furylacryloyl-L-leucinamide
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-
?
additional information
?
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substrate digestion patterns differ from those of atrolysins A and C
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-
?