3.4.24.30: coccolysin
This is an abbreviated version!
For detailed information about coccolysin, go to the full flat file.
Reaction
Preferential cleavage: -/-Leu, -/-Phe, -/-Tyr, -/-Ala =
Synonyms
cell envelope protein, cell wall-anchored proteinase, CEP, EC 3.4.24.4, EM 19000, metalloendopeptidase II, microbial proteinase, PrtS, streptococcal gelatinase, Streptococcus thermophilus intracellular proteinase
ECTree
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Substrates Products on EC 3.4.24.30 - coccolysin
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Ala-Leu-Ile-Leu-Thr-Leu-Val-Ser + H2O
Ala-Leu-Ile-Leu-Thr + Leu-Val-Ser
-
i.e. iPDI, competitive inhibitor of Streptococcus faecalis sex pheromones
-
?
angiotensin I + H2O
Asp-Arg-Val-Tyr + Ile-His-Pro + Phe-His-Leu
-
i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu
-
?
angiotensin II + H2O
Asp-Arg-Val-Tyr + Ile-His-Pro-Phe
-
i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
-
?
Insulin A-chain + H2O
Fragments of insulin A-chain
-
cleavage at Gln15-Leu16 and Tyr14-Gln15, to a much lesser extent than B-chain
peptides (Gln1-Gln15) and (Leu16-Asn21), another product formed: peptide (Gln1-Trp14)
?
insulin B chain + H2O
?
-
rapidly hydrolyzed at Phe24-Phe25, followed by the cleavage of the His5-Leu6 and other bonds
-
-
?
Leu-Phe-Ser-Leu-Val-Leu-Ala-Gly + H2O
Leu-Phe-Ser + Leu-Val-Leu-Ala-Gly
-
i.e. cADI, Streptococcus faecalis sex pheromone, poor substrate
-
?
Leu-Phe-Val-Val-Thr-Leu-Val-Gly + H2O
Leu-Phe-Val-Val-Thr + Leu-Val-Gly
-
i.e. iADI, competitive inhibitor of Streptococcus faecalis sex pheromones
-
?
Leucine-enkephalin + H2O
?
-
cleavage site: Gly3-Phe4, poor substrate
-
-
?
N-3-(2-Furyl)acryloyl tripeptides derivatives + H2O
?
-
chromogenic substrates, e.g. -L-Ala-L-Ala-L-Ala, -Gly-L-Phe-L-Phe, -L-Phe-L-Phe-L-Phe, -Gly-L-Leu-L-Tyr, -Gly-L-Met-L-Leu, -L-Leu-L-Leu-L-Leu
-
-
?
neurotensin + H2O
fragments of neurotensin
-
cleavage site: Tyr11-Ile12, poor substrate
peptides (Arg1-Gly9) and (Leu10-Met11) are the first occuring products
?
Phe-Leu-Val-Met-Phe-Leu-Ser-Gly + H2O
Phe-Leu-Val-Met-Phe + Leu-Ser-Gly
-
i.e. cPDI, Streptococcus faecalis sex pheromone, poor substrate
-
?
Substance P + H2O
Arg-Pro-Lys-Pro-Gln-Gln + Phe + Phe-Gly + Leu-Met-NH2
-
i.e. Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2
-
?
succinyl-Ala-Glu-Pro-Phe-p-nitroanilide + H2O
?
-
-
?
3-methoxysuccinyl-Arg-Pro-Tyr-p-nitroanilide + H2O
?
-
-
?
3-methoxysuccinyl-Arg-Pro-Tyr-p-nitroanilide + H2O
?
-
-
?
acetyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
?
-
-
?
Big endothelin-1 + H2O
?
Enterobacter faecalis
-
human, cleavage sites: Ser5-Leu6 and His16-Leu17
-
-
?
Big endothelin-1 + H2O
?
Enterobacter faecalis OG1-10
-
human, cleavage sites: Ser5-Leu6 and His16-Leu17
-
-
?
Big endothelin-1 fragment(22-38) + H2O
Hydrolyzed fragment of big endothelin fragment
Enterobacter faecalis
-
-
peptides (Val22-Val28) and (Val29-Gly32) and (Leu33-Ser38)
?
Big endothelin-1 fragment(22-38) + H2O
Hydrolyzed fragment of big endothelin fragment
Enterobacter faecalis OG1-10
-
-
peptides (Val22-Val28) and (Val29-Gly32) and (Leu33-Ser38)
?
Bovine fibrinogen + H2O
?
-
B-chain and to a lesser extent A-chain
-
-
?
Endothelin 1 + H2O
?
Enterobacter faecalis
-
presumably involved in initiation and propagation of Enterobacter faecalis associated inflammations, destroys biological activity of endothelin 1
-
-
?
Endothelin 1 + H2O
?
Enterobacter faecalis OG1-10
-
presumably involved in initiation and propagation of Enterobacter faecalis associated inflammations, destroys biological activity of endothelin 1
-
-
?
Human endothelin-1 + H2O
?
Enterobacter faecalis
-
primary cleavage at Ser5-Leu6, subsequent hydrolysis of His16-Leu17, degradation resembles processing by thermolysin, identification of products
-
-
?
Human endothelin-1 + H2O
?
Enterobacter faecalis OG1-10
-
primary cleavage at Ser5-Leu6, subsequent hydrolysis of His16-Leu17, degradation resembles processing by thermolysin, identification of products
-
-
?
Insulin B-chain + H2O
?
-
oxidized, best substrate, preferred cleavage at Phe24-Phe25, followed by His5-Leu6, after prolonged incubation: Ala14-Leu15 and His10-Leu11
-
-
?
N-3-(2-Furyl)acryloyl-Gly-L-Leu amide + H2O
?
-
chromogenic substrate, cleavage site: Gly-Leu, poor substrate
-
-
?
additional information
?
-
-
strict specificity for hydrophobic amino acid at position P1', exhibits endopeptidase and thermolysin-like specificity
-
-
?
additional information
?
-
-
very poor substrates are elastin-orcein or hide powder azure
-
-
?
additional information
?
-
-
No substrates are Ala-Ile-Phe-Ile-Ala-Ser (i.e. Streptococcus faecalis sex pheromone cAM373), aminoacyl-2-naphthylamides, Nalpha-benzoyl-DL-Arg 4-nitroanilide, phenylazobenzyloxycarbonyl-L-Pro-L-Leu-Gly-L-Pro-D-Arg, 2-furylacryloyl-L-Leu-Gly-L-Pro-L-Ala
-
-
?
additional information
?
-
-
No substrates are N-3-(2-furyl)acryloyl-Gly-Gly-Gly, N-3-(2-furyl)acryloyl-Gly-L-Leu or N-3-(2-furyl)acryloyl-Gly-L-Ala
-
-
?
additional information
?
-
-
neutral metalloendopeptidase, no aminopeptidase or esterase activity
-
-
?
additional information
?
-
-
no cleavage of Pz-Pro-Leu-Gly-Pro-D-Arg and 2-FA-Leu-Gly-Pro-Ala
-
-
?
additional information
?
-
Bz-Phe-Val-Arg-p-nitroanilide, NZbz-Gly-Pro-Arg-p-nitroanilide, and Z-Phe-Arg-p-nitroanilide are no substrates
-
?
additional information
?
-
-
Bz-Phe-Val-Arg-p-nitroanilide, NZbz-Gly-Pro-Arg-p-nitroanilide, and Z-Phe-Arg-p-nitroanilide are no substrates
-
?
additional information
?
-
Bz-Phe-Val-Arg-p-nitroanilide, NZbz-Gly-Pro-Arg-p-nitroanilide, and Z-Phe-Arg-p-nitroanilide are no substrates
-
?
