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3.4.24.3: microbial collagenase

This is an abbreviated version!
For detailed information about microbial collagenase, go to the full flat file.

Word Map on EC 3.4.24.3

Reaction

Digestion of native collagen in the triple helical region at -/-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1', Pro and Ala at P2 and P2', and hydroxyproline, Ala or Arg at P3' =

Synonyms

120 kDa collagenase, Achromobacter iophagus collagenase, aspergillopeptidase C, azocollase, bacterial collagenase, bacterial collagenase V, BC_2466, CCA, ChC, class I collagenase, class II collagenase, class III collagenase, clostridial collagenase, clostridial collagenase A, clostridiopeptidase A, clostridiopeptidase I, clostridiopeptidase II, Clostridium histolyticum class II collagenase, Clostridium histolyticum collagenase, ColA, ColG, ColH, collagen peptidase, collagen protease, collagenase, collagenase A, collagenase clostridium histolyticum, collagenase G, collagenase H, collagenase I, collagenase MMP-1, collagenase T, ColT, Dupuytren clostridium histolyticum, Dupuytren collagenase, EC 3.4.24.8, EC 3.4.4.19, EC 3.4.99.5, kollaza, M9-peptidase, matirx metalloproteinase-18, matrix metalloproteinase-1, metallocollagenase, metalloproteinase ColB, metalloproteinase-1, microbial collagenase, MMP-1, More, nucleolysin, peptidase, clostridio-, A, proteinase, Clostridium histolyticum, A, soycollagestin, thermophilic collagenolytic protease, type I collagenase, type II collagenase, VMC peptidase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.3 microbial collagenase

Molecular Weight

Molecular Weight on EC 3.4.24.3 - microbial collagenase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
105000
-
gel filtration
110000
112000
-
ultracentrifugation
112021
sequence calculation, mature enzyme
113000
-
mass spectrometry
113866
-
x * 113866, mass spectrometry, full length enzyme containing two collagen-binding domains
113897
sequence calculation, mature enzyme
114000
1 * 114000, calculated
114200
11470
-
mutant enzyme S906F in presence of 1 mM Ca2+, gel filtration
115000
-
? * 115000, beta, SDS-PAGE
116375
sequence calculation, immature enzyme
11750
-
mutant enzyme S906A in presence of 1 mM Ca2+, gel filtration
11810
-
mutant enzyme Q972A in presence of 1 mM Ca2+, gel filtration
119000
gel filtration, presence of EGTA
11910
-
mutant enzyme V973A in presence of 1 mM Ca2+, gel filtration
11920
-
wild-type enzyme in presence of 1 mM Ca2+, gel filtration
120000
125000
-
? * 125000, zeta, SDS-PAGE
126239
type beta bond, sequence calculation, immature enzyme
13720
-
mutant enzyme E901A in presence of 1 mM Ca2+, gel filtration
13750
-
mutant enzyme D904A in presence of 1 mM Ca2+, gel filtration
14390
-
mutant enzyme E899A in presence of 1 mM Ca2+, gel filtration
147200
-
gel filtration
15370
-
mutant enzyme D930A in presence of 1 mM Ca2+, gel filtration
15420
-
mutant enzyme D930A in presence of 1 mM EDTA, gel filtration
15730
-
mutant enzyme N903A in presence of 1 mM Ca2+, gel filtration
15790
-
mutant enzyme D927A in presence of 1 mM Ca2+, gel filtration
15890
-
mutant enzyme E899A in presence of 1 mM EDTA, gel filtration
16160
-
mutant enzyme D904A in presence of 1 mM EDTA, gel filtration
16190
-
mutant enzyme D927A in presence of 1 mM EDTA, gel filtration
16410
-
mutant enzyme S906A in presence of 1 mM EDTA, gel filtration
16430
-
wild-type enzyme in presence of 1 mM EGTA, gel filtration
16470
-
mutant enzyme Q972A in presence of 1 mM EDTA, gel filtration
16510
-
mutant enzyme V973A in presence of 1 mM EDTA, gel filtration
16530
-
mutant enzyme N903A in presence of 1 mM EDTA, gel filtration
16650
-
mutant enzyme S906F in presence of 1 mM EDTA, gel filtration
24000
-
x * 24000 + x * 28000, SDS-PAGE after denaturation with SDS and beta-mercaptoethanol
28000
-
x * 24000 + x * 28000, SDS-PAGE after denaturation with SDS and beta-mercaptoethanol
35000
-
x * 35000, SDS-PAGE
62000
-
gel filtration
64000
-
1 * 64000, SDS-PAGE
68000
-
? * 68000, alpha, SDS-PAGE
70000
-
gel filtration
72000
-
1 * 72000, IIIb, SDS-PAGE
74000
-
gel filtration
75000
76000
-
? * 76000, C3, SDS-PAGE
79000
-
? * 79000, gamma, SDS-PAGE
81000
-
1 * 81000, I, II, IIIa, SDS-PAGE
82000
gel filtration
90000
-
A2, gel filtration
92000
-
? * 92000, C2, SDS-PAGE
96000
-
? * 96000, C1, SDS-PAGE
additional information
-
MW of various C-terminal segments