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3.4.24.24: gelatinase A

This is an abbreviated version!
For detailed information about gelatinase A, go to the full flat file.

Word Map on EC 3.4.24.24

Reaction

Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-/-Ile-Ala-Gly-Gln =

Synonyms

72 kDa Gelatinase, 72 kDa Gelatinase type A, 72 kDa type IV collagenase, 72-kDa Gelatinase, Collagenase IV, Collagenase type IV, GelA, gelatinase, gelatinase A, human gelatinase A, human matrix metalloproteinase 2, human matrix metalloproteinase-2, LvMMP-2, matrix metallopeptidase 2, matrix metalloprotease 2, Matrix metalloproteinase 2, matrix metalloproteinase-2, matrix metalloprotenase-2, metalloproteinase-2, metrix metalloproteinase-2, MMP 2, MMP-2, MMP2, progelatinase, Type IV collagen metalloproteinase, Type IV collagenase, Type IV collagenase/gelatinase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.24 gelatinase A

Purification

Purification on EC 3.4.24.24 - gelatinase A

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
as an inactive zymogen, rapid activation by 4-aminophenylmercuric acetate
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catalytic domain
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catalytic domain of mutated enzyme
col-1 module
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from pulmonary artery smooth muscle plasma membrane
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native MMP-2 from dentin inculding serial dilutions, method development
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native, extracellular, inhibitor-free enzyme from glioblastoma cell line T98G cell culture medium
purification procedure
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recombinant C domain
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recombinant CBD1, CBD2 and CBD3 polypeptides and fibronectin type-II-like modelues of MMP-2 from Escherichia coli, by gelatin affinity chromatography
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recombinant enzyme
recombinant enzyme from HeLa S3 cells, native enzyme purified from HT-1080 cells
recombinant His-tagged enzyme without pro-domain and isolated MMP-2 CBD domain from Escherichia coli inclusion bodies, after solubilization and refolding, by nickel affinity chromatography
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recombinant pro-gelatinase A
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recombinant proMMP-2 expressed in Sf9 cells
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recombinant soluble enzyme from Escherichia coli cell-free extract by gelatin affinity chromatography
recombinant soluble, refolded His-tagged MMP-2(111-461) from Escherichia coli by nickel affinity chromatography (eluted with 50 mM Tris-HCl, pH 8.0, containing 300 mM NaCl, 4 M urea, and 200 mM imidazole) and dialysis
renaturation of recombinant MMP-2 in truncated form from Escherichia coli strain BL21(DE3) inclusion bodies after nickel affinity chromatography
second fibronectin type II module, residues 278-336, produced in Escherichia coli
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solubilized, refolded recombinant GST-tagged MMP-2 by glutathione affinity chromatography, removal of the tag
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truncated MMP-2 catalytic domain MMP-2C
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with or without fibronectin-like insert
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