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3.4.24.24: gelatinase A

This is an abbreviated version!
For detailed information about gelatinase A, go to the full flat file.

Word Map on EC 3.4.24.24

Reaction

Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-/-Ile-Ala-Gly-Gln =

Synonyms

72 kDa Gelatinase, 72 kDa Gelatinase type A, 72 kDa type IV collagenase, 72-kDa Gelatinase, Collagenase IV, Collagenase type IV, GelA, gelatinase, gelatinase A, human gelatinase A, human matrix metalloproteinase 2, human matrix metalloproteinase-2, LvMMP-2, matrix metallopeptidase 2, matrix metalloprotease 2, Matrix metalloproteinase 2, matrix metalloproteinase-2, matrix metalloprotenase-2, metalloproteinase-2, metrix metalloproteinase-2, MMP 2, MMP-2, MMP2, progelatinase, Type IV collagen metalloproteinase, Type IV collagenase, Type IV collagenase/gelatinase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.24 gelatinase A

Cloned

Cloned on EC 3.4.24.24 - gelatinase A

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA cloned and expressed in Escherichia coli JM-109
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cDNA of the mutated enzyme used to transfect HEK 293 cells
cDNA, recombinant C domain expressed in Escherichia coli
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cloned and expressed in Escherichia coli
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cloned and overexpressed in a bacterial system, Escherichia coli BL21(DE3)pLysS
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expression of GST-tagged MMP-2 in Escherichia coli strain DH5alpha in inclusion bodies
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expression of His- and myc-tagged pro-MMP-2
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expression of the first, second, and third module, i.e. CBD1, CBD2 and CBD3 polypeptides, and of the fibronectin type-II-like modelues of MMP-2 in Escherichia coli
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expression of the His-tagged enzyme without pro-domain and of the isolated MMP-2 CBD domain in Escherichia coli in inclusion bodies
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full-length progelatinase A
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gene LvMMP-2, DNA and amino acid sequence determination and analysis, sequence compariosns and phylogenetic analysis, quantitative real-time RT-PCR enzyme expression analysis
gene MMP-2 , cloning from skeletal muscle, DNA and amino acid sequence determination and analysis, sequence comparissons, expression of recombinant His-tagged MMP-2 in truncated form in Escherichia coli inclusion bodies
gene MMP-2, quantitative real time PCR expression analysis in glioblastoma
gene MMP2, genotyping in Tunesian population. The levels of MMP-2 mRNA expression in patients containing the CC genotype are much higher compared with cells with the CT genotype. The frequency of the MMP-2 CC genotype is significantly higher in colorectal cancer patients when compared with controls
gene MMP2, quantitative RT-PCR enzyme expression analysis
gene MMP2, sequence comparisons, enzyme expression analysis
gene MMP2, the gene is located on the long arm of chromosome 16 at position q12.2, the cDNA for MMP-2 codes for a 660 residues preproenzyme containing a 29 residues signal peptide responsible for translocation to the endoplasmic reticulum and followed by the 72-kDa-proenzyme. The human MMP2 gene lacks a conventional TATA-box or classical transcription response elements found in other MMP genes, the constitutive expression depends on p53-, Sp1-, Sp3-and AP-2-binding sites but the promoter contains other cis-regulatory elements including C/EBP, ATF2, PEA3/Ets, c-myc and AP-1 binding sites. Transcriptionally active MMP-2 in invasive cancer cells is characterized by hypomethylation of CpG regions in the MMP2 promoter and low levels of histone H3 lysine-27 trimethylation
MMP-2 expression analysis in embryonic tissues of wild-type and caireticulin-deficient mice, overview
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MMP-2 expression analysis in SK-Hep1 hepatoma cells, overview
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MMP-2 gene cloning from the body wall of sea cucumber, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged MMP-2(111-461) comprising the catalytic domain in Escherichia coli strain BL21(DE3) in inclusion bodies
pro-gelatinase A in transfected mammalian cell lines
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pro-MMP-2 expressed in a recombinant vaccinia virus mammalian cell expression system
recombinant enzyme from HeLa S3 cells infected with vaccinia virus encoding the full-length cDNA of pro-MMP-2
recombinant expression of soluble and functional full-length enzyme from plasmid pET5a-MMP-2 in the cytoplasm of Escherichia coli strain BL21(DE3)/pLysS, subcloning in Escherichia coli strain DH5alpha, method development and evaluation, overview
recombinant expression of wild-type and mutant full-length MMP-2 or MMP-2 with C-terminal Myc and His tags in COS-1 cells, secretion of the enzyme to the cell culture medium
recombinant proMMP-2 expressed in Sf9 cells
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second fibronectin type II module, residues 278-336, produced in Escherichia coli
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truncated MMP-2 catalytic domain MMP-2C
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