3.4.24.23: matrilysin

This is an abbreviated version!
For detailed information about matrilysin, go to the full flat file.

Reaction

Cleavage of Ala14-/-Leu and Tyr16-/-Leu in B chain of insulin. No action on collagen types I, II, IV, V. Cleaves gelatin chain alpha2(I) > alpha1(I) =

Synonyms

cyclic matrix metalloproteinase, human matrix metalloproteinase 7, matrilysin, matrilysin 1, matrilysin-1, Matrin, matrix metallopeptidase 7, matrix metalloprotease-7, Matrix metalloproteinase 7, Matrix metalloproteinase pump 1, Matrix metalloproteinase-7, matrix-metalloproteinase-7, metalloproteinase-7, MMP, MMP 7, MMP-7, MMP7, More, Proteinase, metallo-, pump-1, Proteinase, PUMP-1, PUMP, PUMP-1, Pump-1 protease, Putative (or punctuated) metalloproteinase-1, Putative metalloproteinase, Uterine metalloendopeptidase, Uterine metalloproteinase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.24 Metalloendopeptidases

                3.4.24.23 matrilysin

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Results	in table
17	Activating Compound
13	AA Sequence
31	Application
1	CAS Registry Number
35	Cloned(Commentary)
3	Crystallization (Commentary)
7149	Disease/ Diagnostics
25	Expression
84	General Information
4	IC50 Value
190	Inhibitors
6	kcat/KM [mM/s]
24	Ki Value [mM]
24	KM Value [mM]
62	Localization
50	Metals/Ions
13	Molecular Weight [Da]
79	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
524	Organism
13	PDB ID
24	pH Optimum
5	pH Range
14	Posttranslational Modification
47	Protein Variants
16	Purification (Commentary)
1	Reaction
1	Reaction Type
174	Reference
4	Renatured (Commentary)
272	Source Tissue
3	Specific Activity [micromol/min/mg]
215	Substrates and Products (Substrate)
10	Subunits
252	Synonyms
23	Temperature Optimum [°C]
2	Temperature Range [°C]
5	Temperature Stability [°C]
21	Turnover Number [1/s]

KM Value

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KM Value on EC 3.4.24.23 - matrilysin

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.019 - 0.057

(7-methoxycoumarin-4-yl)-acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2

2 entries

0.028

(7-methoxycoumarin-4-yl)acetyl-L-Pro-Leu-Gly-L-Leu-[N3-2,4-dinitrophenyl-L-2,3-diamino-propionyl]-L-Ala-L-Arg-NH2

Homo sapiens

-

pH 7.5, 25°C

651777

0.0511 - 0.211

(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2

6 entries

0.026

2,4-Dinitrophenyl-Arg-Pro-Leu-Ala-Leu-Trp-Arg-Ser

Homo sapiens

-

-

31080

0.065

2,4-dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg

Homo sapiens

-

-

31080

0.0079 - 0.024

dansyl-PLALWAR

4 entries

0.00089

entactin

Mammalia

-

-

31071

-

0.81

Gly-Pro-Gln-Ala-Ile-Ala-Gly-Gln

Homo sapiens

-

-

31070

2

Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln

Homo sapiens

-

-

31070

2.3

Gly-Pro-Gln-Gly-Ile-Ala-Met-Gln

Homo sapiens

-

-

31070

7.3

Gly-Pro-Gln-Gly-Leu-Ala-Gly-Gln

Homo sapiens

-

-

31070

4.2

Gly-Pro-Met-Gly-Ile-Ala-Gly-Gln

Homo sapiens

-

-

31070

0.0005

PB-M7vis

Mus musculus

-

25°C, pH 7.4

667466

-

additional information

additional information

2 entries

-

0.019

(7-methoxycoumarin-4-yl)-acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2

Homo sapiens

P09237

pH 7.5, 25°C, with 0.05 mM heparin

733534

0.057

(7-methoxycoumarin-4-yl)-acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2

Homo sapiens

P09237

pH 7.5, 25°C, without heparin

733534

0.0511

(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2

Homo sapiens

-

pH 7.5, 25°C, mutant Y219C

717745

0.0732

(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2

Homo sapiens

-

pH 7.5, 25°C, mutant Y219F

717745

0.0736

(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2

Homo sapiens

-

pH 7.5, 25°C, wild-type enzyme

717745

0.0788

(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2

Homo sapiens

-

pH 7.5, 25°C, mutant Y219A

717745

0.0865

(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2

Homo sapiens

-

pH 7.5, 25°C, mutant Y219D

717745

0.211

(7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2

Homo sapiens

-

pH 7.5, 25°C, mutant Y219S

717745

0.0079

dansyl-PLALWAR

Homo sapiens

-

pH 7.5, 25°C, mutant E198A

649827

0.018

dansyl-PLALWAR

Homo sapiens

-

pH 7.5, 25°C, mutant E198C and E198Q

649827

0.021

dansyl-PLALWAR

Homo sapiens

-

pH 7.5, 25°C, mutant E198D

649827

0.024

dansyl-PLALWAR

Homo sapiens

-

pH 7.5, 25°C, wild-type

649827

additional information

additional information

Mammalia

-

highly active and selective substrates produced by using bacteriophage peptide display libraries

31078

-

additional information

additional information

Homo sapiens

-

Km-value decreases from 2.57 mg/ml to 2.29 mg/ml upon nitration of 4-5 Tyr residues of enzyme, pH 7.5, 25°C

667492

-