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3.4.24.21: astacin

This is an abbreviated version!
For detailed information about astacin, go to the full flat file.

Word Map on EC 3.4.24.21

Reaction

Hydrolysis of peptide bonds in substrates containing five or additional information amino acids, preferentially with Ala in P1', and Pro in P2' =

Synonyms

Ace-MTP-2, Ancylostoma ceylanicum metalloprotease 2, Ast1, Ast2, Ast3, Ast4, Ast5, Ast6, Ast7, Ast9, astacin, astacin metallopeptidases, astacin metalloprotease, astacin-like metalloendopeptidase, astacin-like metalloprotease, astacin-like metalloprotease toxin 3, astacin-Like protease 3, Astacus protease, Astacus proteinase, ASTL, BMP-1, bone morphogenetic protein 1, carp nephrosin, crayfish small-molecule protease, Crayfish small-molecule proteinase, DPY-31, EC 3.4.99.6, LALP3, LAST, LAST_MAM, MEP1A, MEP1B, More, NAS-35, nas-36, nas-37, nephrosin, Ovastacin, Procollagen C-proteinase, Qcam1, Sc-AST, Smed-ast-1, Smed-ast-2, Smed-ast-3, Smed-ast-4, Smed-ast-5, Smed-ast-6, Smed-ast-7, Smed-ast-9, toxin 3, Xhe2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.21 astacin

Cloned

Cloned on EC 3.4.24.21 - astacin

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
astacins genomic organization, phylogenetic analysis
astacins genomic organization, phylogenetic analysis, enzyme expression analysis during regeneration, overview
astacins genomic organization, phylogenetic analysis, enzyme expression analysis in tissues and during regeneration
cDNA reverse-transcribed from midgut-gland mRNA, cloned into pET3a vector and used to transform Escherichia coli BL21(DE3)
-
expressed in Escherichia coli BL21(DE3) cells
expression in Hi5 cell
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gene Ace-mtp-2, DNA and amino acid seuence determination and analysis, recombinant expression in Escherichia coli strains BL21 and Rosetta and Pichia pastoris strain X33
gene ASTL, sequence comparisons
gene BMP1, sequence comparison with meprin A (EC 3.4.24.18) and meprin B (EC 3.4.24.63) of the astacin enzyme family
gene dpy-31, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis
gene qcam1, sequence comparisons
gene xh2, DNA and amino acid sequence determination and analysis, sequence comparison, expression analysis
recombinantly expressed as a His-tagged fusion in Escherichia coli
recombinantly expressed in Escherichia coli
sequence comparisons and phylogenetic tree, recombinant expression of SUMO-His6-tagged enzyme in Escherichia coli strain SHuffle T7 Express LysY