3.4.24.15: thimet oligopeptidase

This is an abbreviated version!
For detailed information about thimet oligopeptidase, go to the full flat file.

Reaction

Preferential cleavage of bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5-15 residues =

Synonyms

BIE, bradykinin-inactivating endopeptidase, EC 3.4.22.19, EC 3.4.99.31, endo-oligopeptidase A, endooligopeptidase A, endopeptidase 24-15, endopeptidase 24.15, endopeptidase EC 3.4.24.15, EOPA, EP 24.15, EP24.15, MdpA, metallo-dipeptidase aeruginosa, metalloendopeptidase 24.15, metalloendopeptidase EC 3.4.24.15, metallopeptidase, More, MP78, neutral endopeptidase 24.15, PA4498, peptidase, thimet oligo-, Pz peptidase A, Pz peptidase B, Pz-peptidase, Pz-peptidase A, soluble metallo-endopeptidase, soluble metallopeptidase, thimet oligopeptidase, thimet peptidase, thimet-oligopeptidase, thiol-dependent metalloendopeptidase, THOP1, TOP, Top1, Top2

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.24 Metalloendopeptidases

                3.4.24.15 thimet oligopeptidase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
16	Activating Compound
165	AA Sequence
1	Application
1	CAS Registry Number
26	Cloned(Commentary)
6	Crystallization (Commentary)
948	Disease/ Diagnostics
6	Expression
46	General Information
2	General Stability
20	IC50 Value
171	Inhibitors
20	kcat/KM [mM/s]
31	Ki Value [mM]
242	KM Value [mM]
60	Localization
39	Metals/Ions
17	Molecular Weight [Da]
66	Natural Substrates/ Products (Substrates)
296	Organism
2	Oxidation Stability
2	PDB ID
15	pH Optimum
5	pH Range
2	pH Stability
5	Posttranslational Modification
30	Protein Variants
20	Purification (Commentary)
1	Reaction
2	Reaction Type
89	Reference
1	Renatured (Commentary)
119	Source Tissue
12	Specific Activity [micromol/min/mg]
1	Storage Stability
417	Substrates and Products (Substrate)
21	Subunits
91	Synonyms
9	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
252	Turnover Number [1/s]

Purification

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Purification on EC 3.4.24.15 - thimet oligopeptidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Purification (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

6 entries

native enzyme from brain

Mus musculus

-

683961

recombinant enzyme from Escherichia coli strain BL21 (DE3) by anion exchange chromatography and gel filtration

Geobacillus sp.

Q4W802, Q4W803

733022

recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography to over 95% homogeneity, the protein is released from the GST fusion by cleavage with thrombin

Homo sapiens

P52888

735106

recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain DH5alpha by glutathione affinity chromatography to homogeneity

Rattus norvegicus

-

683138

recombinant His-tagged enzyme TOP1 from Escherichia coli strain Bl21 by cobalt affinity chromatography and gel filtration

Arabidopsis thaliana

A0A1R7T3A4

753710

recombinant His-tagged enzyme TOP2 from Escherichia coli strain Bl21 by cobalt affinity chromatography and gel filtration

Arabidopsis thaliana

A0A1R7T3A4

753710

recombinant Pz peptidase A from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography

Geobacillus sp.

-

683026

recombinant Pz peptidases A and B from Escherichia coli strain BL21(DE3) by heat denaturation, anion exchange chromatography and gel filtration, 2.2fold and 8.4fold, respectively

Geobacillus sp.

-

683273

recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)

Homo sapiens

-

683672

-

Gallus gallus

-

37055

-

Homo sapiens

-

37054, 37063, 652533

-

Rattus norvegicus

-

37053, 37057, 37067

-

Sus scrofa

-

37053, 37062

-

Bos taurus

-

37066

-

Bacillus licheniformis

-

37060