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3.4.24.15: thimet oligopeptidase

This is an abbreviated version!
For detailed information about thimet oligopeptidase, go to the full flat file.

Word Map on EC 3.4.24.15

Reaction

Preferential cleavage of bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5-15 residues =

Synonyms

BIE, bradykinin-inactivating endopeptidase, EC 3.4.22.19, EC 3.4.99.31, endo-oligopeptidase A, endooligopeptidase A, endopeptidase 24-15, endopeptidase 24.15, endopeptidase EC 3.4.24.15, EOPA, EP 24.15, EP24.15, MdpA, metallo-dipeptidase aeruginosa, metalloendopeptidase 24.15, metalloendopeptidase EC 3.4.24.15, metallopeptidase, More, MP78, neutral endopeptidase 24.15, PA4498, peptidase, thimet oligo-, Pz peptidase A, Pz peptidase B, Pz-peptidase, Pz-peptidase A, soluble metallo-endopeptidase, soluble metallopeptidase, thimet oligopeptidase, thimet peptidase, thimet-oligopeptidase, thiol-dependent metalloendopeptidase, THOP1, TOP, Top1, Top2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.15 thimet oligopeptidase

Cloned

Cloned on EC 3.4.24.15 - thimet oligopeptidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2.3 kb cDNA coding for a truncated enzyme of 512 amino acids, displaying the same enzymatic features as endooligopeptidase A
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cloned from testis and brain
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expressed in HeLa cells and in Escherichia coli
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expression analysis by quantitative real-time PCR
expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain DH5alpha
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expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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expression of N-terminally HA- or FLAG-tagged enzyme in HEK-293 cells, subcellular localization in the transformed cells, overview
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expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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for crystallization, recombinant Pz-peptidase A is purified from an Escherichia coli strain BL21
gene PA4498, DNA and amino acid sequence determination and analysis
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gene Thop1, genotyping, enzyme expression analysis
gene Thop1, recombinant expression of wild-type and mutant enzymes
large-scale expression in Escherichia coli
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overexpression in Escherichia coli
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overexpression of Pz peptidase A in Escherichia coli strain BL21 (DE3)
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Pz peptidases A and B overexpression in Escherichia coli strain BL21(DE3)
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recombinant enzyme expression in Escherichia coli strain BL21 (DE3)
recombinant enzyme from testis, expression in HEK-293 cell
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recombinant expression of GST-tagged enzyme in Escherichia coli
recombinant expression of His-tagged enzyme TOP1 in Escherichia coli strain Bl21. Arabidopsis protoplasts are prepared and co-transformed with pairs of plasmid constructs containing the coding sequences of TOP1 or TOP2, cloned in frame with either the N-terminal or the C-terminal halves of the Renilla luciferase coding sequence (TOP1-orTOP2-NLuc and TOP1- or TOP2- CLuc). Interactions between TOPs-Luc fusions are detected by measuring luminescence released upon the restoration of luciferase enzymatic activity. Protoplasts expressing luciferase terminal fusions with known interactors MKK5 and HOPF2
recombinant expression of His-tagged enzyme TOP2 in Escherichia coli strain Bl21. Arabidopsis protoplasts are prepared and co-transformed with pairs of plasmid constructs containing the coding sequences of TOP1 or TOP2, cloned in frame with either the N-terminal or the C-terminal halves of the Renilla luciferase coding sequence (TOP1-orTOP2-NLuc and TOP1- or TOP2- CLuc). Interactions between TOPs-Luc fusions are detected by measuring luminescence released upon the restoration of luciferase enzymatic activity. Protoplasts expressing luciferase terminal fusions with known interactors MKK5 and HOPF2