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3.4.24.11: neprilysin

This is an abbreviated version!
For detailed information about neprilysin, go to the full flat file.

Word Map on EC 3.4.24.11

Reaction

preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1' =

Synonyms

Abeta-degrading enzyme, acute lymphoblastic leukemia antigen, antigen, CALLA (common acute lymphoblastic leukemia-associated), atriopeptidase, CALLA, CALLA (common acute lymphoblastic leukemia-associated) antigens, CALLA antigen, CALLA glycoproteins, CD10, CD10/neutral endopeptidase, CD10/neutral endopeptidase 24.11, common acute lymphoblastic leukemia antigen, common acute lymphoblastic leukemia-associated antigens, Common acute lymphocytic leukemia antigen, endopeptidase CD10, Endopeptidase-2, endopeptidase-24.11, enkephalinase, EP24.11, glycoprotein, CALLA, kidney-brush-border neutral endopeptidase, kidney-brush-border neutral peptidase, kidney-brush-border neutral proteinase, membrane metallo-endopeptidase, membrane metalloendopeptidase, MME, NEP, NEP 24.11, NEP, enkephalinase, neutrophil cluster-differentiation antigen 10, common acute lymphoblastic leukemia antigen, NEP-1, NEP/CD10, NEP2, NEP4A, NEP4B, neprilypsin, neprilysin, neprilysin 4, neutral endopeptidase, neutral endopeptidase 24.11, neutral endopeptidase 24.11/CD10, neutral metallendopeptidase, NL-1, peptidase, endo-, peptidase, membrane metalloendo-, SEP, skin fibroblast elastase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.11 neprilysin

KM Value

KM Value on EC 3.4.24.11 - neprilysin

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0068 - 0.0224
(7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(2,4-dinitrophenyl)
0.0687
2-aminobenzoyl-ARFK-2,4-dinitrophenyl ester
-
-
0.0178
2-aminobenzoyl-DRFK-2,4-dinitrophenyl ester
-
-
0.0097
2-aminobenzoyl-FRFK-2,4-dinitrophenyl ester
-
-
0.0161
2-aminobenzoyl-HRFK-2,4-dinitrophenyl ester
-
-
0.0093
2-aminobenzoyl-IRFK-2,4-dinitrophenyl ester
-
-
0.0528
2-aminobenzoyl-KRFK-2,4-dinitrophenyl ester
-
-
0.003
2-aminobenzoyl-LRFK-2,4-dinitrophenyl ester
-
-
0.0214
2-aminobenzoyl-NRFK-2,4-dinitrophenyl ester
-
-
0.0117
2-aminobenzoyl-RAFK-2,4-dinitrophenyl ester
-
-
0.0028
2-aminobenzoyl-RDFK-2,4-dinitrophenyl ester
-
-
0.0043
2-aminobenzoyl-REFK-2,4-dinitrophenyl ester
-
-
0.054
2-aminobenzoyl-rGF-N-(2,4-dinitrophenyl)ethylenediamine
-
-
0.0259
2-aminobenzoyl-RGFK-2,4-dinitrophenyl amide
-
-
0.014
2-aminobenzoyl-RGFK-2,4-dinitrophenyl ester
-
-
0.0273
2-aminobenzoyl-rGL-N-(2,4-dinitrophenyl)ethylenediamine
-
-
0.0089
2-aminobenzoyl-RHFK-2,4-dinitrophenyl ester
-
-
0.9981
2-aminobenzoyl-RKFK-2,4-dinitrophenyl ester
-
-
0.0024
2-aminobenzoyl-RNFK-2,4-dinitrophenyl ester
-
-
0.0075
2-aminobenzoyl-RPFK-2,4-dinitrophenyl ester
-
-
0.0121
2-aminobenzoyl-RQFK-2,4-dinitrophenyl ester
-
-
0.035
2-aminobenzoyl-RRFK-2,4-dinitrophenyl amide
-
-
0.0089
2-aminobenzoyl-RRFK-2,4-dinitrophenyl ester
-
-
0.028
2-aminobenzoyl-rRL-N-(2,4-dinitrophenyl)ethylenediamine
-
-
0.0084
2-aminobenzoyl-RSFK-2,4-dinitrophenyl ester
-
-
0.0259
2-aminobenzoyl-rSL-N-(2,4-dinitrophenyl)ethylenediamine
-
-
0.0108
2-aminobenzoyl-RTFK-2,4-dinitrophenyl ester
-
-
0.0081
2-aminobenzoyl-SRFK-2,4-dinitrophenyl ester
-
-
0.0095
2-aminobenzoyl-TRFK-2,4-dinitrophenyl ester
-
-
0.0109
2-aminobenzoyl-VRFK-2,4-dinitrophenyl ester
-
-
0.051
2-aminobenzoyl-WRFK-2,4-dinitrophenyl ester
-
-
0.0583
2-aminobenzoyl-YRFK-2,4-dinitrophenyl ester
-
-
0.0028
adrenocorticotropic hormone
-
-
-
0.109
Ala-Leu-enkephalin
-
wild type enzyme
0.024
alpha-endorphin
wild-type enzyme, pH and temperature not specified in the publication
0.135
alpha-neoendorphin
-
-
0.014 - 0.104
amyloid beta peptide1-40
-
0.0086
angiotensin
wild-type enzyme, pH and temperature not specified in the publication
0.0004 - 34.1
angiotensin I
0.179
angiotensin II
-
37°C, pH 7.4
0.1114
angiotensin(1-9)
-
37°C, pH 7.4
0.0062
Arg-vasopressin
wild-type enzyme, pH and temperature not specified in the publication
0.25
benzyloxycarbonyl-Ala-Gly-Leu-Ala
-
-
0.00654
beta-endorphin
-
-
0.005 - 0.162
bradykinin
0.296
cholecystokinin-8
-
-
0.07 - 2.137
D-Ala2-Leu5-enkephalin
0.773
D-Ala2-Leu5-enkephalinamide
-
-
0.432
D-Ala2-Met5-enkephalinamide
-
-
0.03
dansyl-Gly-Trp-Gly
-
-
0.041
dansyl-Gly-Tyr-Gly
-
-
0.09
dansyl-Gly-Tyr-Gly-NH2
-
-
0.035
dynorphin A-10
-
-
0.01
dynorphin A-13
-
-
0.0082
dynorphin A-17
-
-
0.025
dynorphin A-6
-
-
0.298
dynorphin A-8
-
-
0.04
dynorphin A-9
-
-
0.119
dynorphin(1-9)
-
-
0.053
endothelin-1
wild-type enzyme, pH and temperature not specified in the publication
0.013
gamma-endorphin
wild-type enzyme, pH and temperature not specified in the publication
0.15
gastri-releasing peptide
wild-type enzyme, pH and temperature not specified in the publication
0.049 - 0.068
gastrin G-17
0.024
GLP-1
wild-type enzyme, pH and temperature not specified in the publication
-
0.025
glucagon
wild-type enzyme, pH and temperature not specified in the publication
0.59
glutaryl-Ala-Ala-Phe-2-naphthylamide
-
-
0.12
glutaryl-Ala-Ala-Phe-4-methoxy-2-naphthylamine
-
-
0.0685
glutaryl-Gly-Gly-Phe-2-naphthylamide
-
-
0.38
hippuryl-Arg-Arg-Ala-2-naphthylamide
-
-
0.35
hippuryl-Arg-Arg-Leu-2-naphthylamide
-
-
0.133
His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2
-
-
0.074 - 0.2
Leu-enkephalin
0.612
Leu-enkephalinamide
-
-
0.086
Leu5-enkephalin
-
-
0.111
Leu5-enkephalin-Arg6
-
-
0.68
Leu5-enkephalinamide
-
-
0.755
Luteinizing hormone-releasing hormone
0.022 - 0.1
Met-enkephalin
0.41
Met-enkephalin amide
-
-
0.062
Met5-enkephalin
-
-
0.039
Met5-enkephalin-Arg6
-
-
0.05
Met5-enkephalin-Arg6Phe7
-
-
0.011 - 0.087
N-(4-carboxy-1-oxobutyl)-L-alanyl-L-alanyl-N-(4-methoxy-2-naphthalenyl)-L-phenylalaninamide
0.42
N-(4-carboxybutanoyl)-L-alanyl-L-alanyl-N-(4-methoxynaphthalen-2-yl)-L-phenylalaninamide
-
-
0.0408
N-2-aminobenzoyl-rGV-N-(2,4-dinitrophenyl)ethylenediamine
-
-
0.13
N-benzyoxycarbonyl-Gly-Gly-Leu 2-naphthylamide
-
-
0.3
Nalpha-benzoyl-Gly-Arg-Arg-Ala-2-naphthylamide
-
-
0.18
Nalpha-benzoyl-Gly-Arg-Arg-Leu-2-naphthylamide
-
-
0.11
Nalpha-benzoyl-Gly-Arg-Arg-Phe-2-naphthylamide
-
-
0.19
Nalpha-benzoyl-Gly-Arg-Leu-2-naphthylamide
-
-
0.11
Nalpha-benzoyl-Gly-Gly-Arg-Leu-2-naphthylamide
-
-
0.08
Nalpha-benzoyl-Gly-Lys-Arg-Arg-Leu-2-naphthylamide
-
-
0.03
Nalpha-benzoyl-Gly-Lys-Lys-Arg-Arg-Leu-2-naphthylamide
-
-
0.0093
Neurokinin A
wild-type enzyme, pH and temperature not specified in the publication
0.022
neurokinin B
wild-type enzyme, pH and temperature not specified in the publication
0.016 - 0.078
neurotensin
0.02
nociceptin
wild-type enzyme, pH and temperature not specified in the publication
0.106
Physalaemin
-
-
0.0074
somatostatin 14
wild-type enzyme, pH and temperature not specified in the publication
0.031
somatostatin 28
wild-type enzyme, pH and temperature not specified in the publication
0.01 - 0.19
Substance P
0.043 - 0.073
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide
0.058
succinyl-Ala-Ala-Phe-AMC
-
plasma enzyme
0.45
succinyl-Arg-Arg-Leu-2-naphthylamide
-
-
0.057
sulfated cholecystokinin octapeptide
-
-
0.018 - 0.019
Tyr-D-Ala-Gly-Phe-Leu
0.013
Tyr-D-Ala-Gly-Phe-Met
-
-
0.061 - 0.32
Tyr-D-Ala-Gly-Phe-met-NH2
0.008
Tyr-Gly-Gly-Phe-Met
-
-
0.033
unsulfated cholecystokinin octapeptide
-
-
0.0018
Vasoactive intestinal peptide
-
-
additional information
additional information
Michaelis-Menten profiles and steady-state kinetic parameters for cleavage of amyloid beta peptide1-40 and off-target peptide by wild-type and mutant enzymes, overview
-