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3.4.24.11: neprilysin

This is an abbreviated version!
For detailed information about neprilysin, go to the full flat file.

Word Map on EC 3.4.24.11

Reaction

preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1' =

Synonyms

Abeta-degrading enzyme, acute lymphoblastic leukemia antigen, antigen, CALLA (common acute lymphoblastic leukemia-associated), atriopeptidase, CALLA, CALLA (common acute lymphoblastic leukemia-associated) antigens, CALLA antigen, CALLA glycoproteins, CD10, CD10/neutral endopeptidase, CD10/neutral endopeptidase 24.11, common acute lymphoblastic leukemia antigen, common acute lymphoblastic leukemia-associated antigens, Common acute lymphocytic leukemia antigen, endopeptidase CD10, Endopeptidase-2, endopeptidase-24.11, enkephalinase, EP24.11, glycoprotein, CALLA, kidney-brush-border neutral endopeptidase, kidney-brush-border neutral peptidase, kidney-brush-border neutral proteinase, membrane metallo-endopeptidase, membrane metalloendopeptidase, MME, NEP, NEP 24.11, NEP, enkephalinase, neutrophil cluster-differentiation antigen 10, common acute lymphoblastic leukemia antigen, NEP-1, NEP/CD10, NEP2, NEP4A, NEP4B, neprilypsin, neprilysin, neprilysin 4, neutral endopeptidase, neutral endopeptidase 24.11, neutral endopeptidase 24.11/CD10, neutral metallendopeptidase, NL-1, peptidase, endo-, peptidase, membrane metalloendo-, SEP, skin fibroblast elastase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.11 neprilysin

Cloned

Cloned on EC 3.4.24.11 - neprilysin

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
3 human cDNA types result from alternative splicing of exons 1, 2a, or 2b to the common exon 3
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cloned into the pQE expression vector, resulting in the fusion of a hexahistidine at the N-terminus, expression in Escherichia coli
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cloning of the extracellular enzyme domain (52-750) with a is-tag and a gp64 signal sequence in pBAC-6 expression vector in Spodoptera frugiperda insect cells and recombinant expression of the His-tagged enzyme in insect cells and in Alzheimer disease mice using intracerebral injection of the NEP-expression vector. Accumulation of amyloid beta is significantly reduced and the behavioral performance of the mice is improved, phenotype, overview
expressed as a GST-fusion protein in Escherichia coli
Q9I7I4
expression in 3xTg-AD mice containing, in addition to a PS1M146V knock-in gene, a human APP695 gene carrying the Swedish double mutation and a human four-repeat tau harboring the P30lL mutation
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expression in amyloid precursor protein transgenic mice
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expression in mouse brain
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expression of N-terminally FLAG-tagged wild-type and mutant enzymes in HEK-293 cell membranes
generation of both neprilysin and amyloid precursor protein transgenic mice
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overexpression in transgenic mice
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quantitative real-time PCR enzyme expression analysis
recombinant enzyme expression in HEK-293 cells
recombinant expression in HEK-293T cells
recombinant expression of C-terminally Strep-tagged neprilysin residues Gly52-Trp749 with a cathepsin D leader sequence, comprising the extracellular domain of NEP, in Spodoptera frugiperda cells using the baculovirus transfection system
recombinant expression of His10-tagged wild-type and mutant enzymes in CHO suspension cells, recombinant expression of HA-tagged wild-type and mutant enzymes in Saccharomyces cerevisiae strain YMR307w
recombinant expression of the N-terminally His6-tagged extracellular catalytic domain, NEP(Tyr51-Trp749), in Pichia pastoris strain GS115. The cytoplasmic (Gly1-Glu27) and transmembrane (Ile28-Ala50) domains of NEP are absent in the construct
recombinant expression of wild-type and mutant C-terminally human serum albumin-fused extracellular domains of human NEP (amino acids 52-749) in CHO cells
recombinant expression of wild-type and mutant enzymes in HEK-293T cells
recombinant viral expression of NEP in primary neurons
recombinantly expressed
transgenic Drosophila melanogaster expressing human neprilysin and amyloid beta42
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