3.4.23.B8: human T-cell leukemia virus type 1 protease

This is an abbreviated version!
For detailed information about human T-cell leukemia virus type 1 protease, go to the full flat file.

Reaction

Processing at the authentic HIV-1 PR recognition site and release of the mature p17 matrix and the p24 capsid protein, as a result of the cleavage of the -SQNY-/-PIVQ- cleavage site. =

Synonyms

C2A HTLV-1 PR, HTLV-1 PR, HTLV-1 protease, HTLV-I PR, HTLV-I protease, human T cell leukemia virus PR, human T cell leukemia virus protease, human T-cell leukemia virus type 1 protease, human T-lymphotropic virus type I protease, More, retropepsin (human T-cell leukemia virus), simian T-cell leukemia virus endopeptidase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.23 Aspartic endopeptidases

                3.4.23.B8 human T-cell leukemia virus type 1 protease

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Results	in table
2	AA Sequence
4	Application
1	CAS Registry Number
9	Cloned(Commentary)
8	Crystallization (Commentary)
6	General Information
68	IC50 Value
164	Inhibitors
11	kcat/KM [mM/s]
39	Ki Value [mM]
61	KM Value [mM]
1	Metals/Ions
5	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
38	Organism
5	pH Optimum
1	pH Range
1	Posttranslational Modification
25	Protein Variants
6	Purification (Commentary)
1	Reaction
1	Reaction Type
38	Reference
2	Renatured (Commentary)
2	Specific Activity [micromol/min/mg]
1	Storage Stability
130	Substrates and Products (Substrate)
10	Subunits
27	Synonyms
5	Temperature Optimum [°C]
66	Turnover Number [1/s]

KM Value

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KM Value on EC 3.4.23.B8 - human T-cell leukemia virus type 1 protease

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.058

(((4-((4-dimethylamino)phenyl)azo)phenyl)sulfonyl)amino-(gamma-aminobutyric acid)-PQVL-F(NO2)-VMH-(5-(2-aminoethylamino)-1-naphthalenesulfonic acid)

Human T-cell leukemia virus type I

-

pH 5.2, 37°C

647861

0.06

(((4-((4-dimethylamino)phenyl)azo)phenyl)sulfonyl)amino-(gamma-aminobutyric acid)-PQVL-PVMH-(5-(2-aminoethylamino)-1-naphthalenesulfonic acid)

Human T-cell leukemia virus type I

-

pH 5.2, 37°C

647861

0.055

4-((4-dimethylaminophenylazo)benzoyl)-(gamma-aminobutyric acid)-PQVL-(4-nitro)Phe-VMH-(5-(2-aminoethylamino)-1-naphthalenesulfonic acid)

Human T-cell leukemia virus type I

-

pH 5.2, 37°C

647861

0.031 - 0.047

Ala-Pro-Gln-Val-Sta-Val-Met-His-Pro-Leu

3 entries

0.042 - 0.062

APQVLF(NO2)VMHPL

2 entries

0.061

APQVLNphVMHPL

Human T-cell leukemia virus type I

-

pH 5.3, 37°C

647851

0.098 - 0.8

APQVLPVMHP

4 entries

0.3 - 0.47

APQVLPVMHPHG

3 entries

0.0068 - 0.01

dabsyl-(GABA)-PQVLPVMH-EDANS

2 entries

0.23 - 0.244

DPASILPVIP

3 entries

0.086

KAKVLVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.48

KDKVLVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.069

KGKVLVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.03 - 0.034

KGPPVILPIQAP

2 entries

0.23

KSKVLVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.18

KTDVLVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.13

KTFVLVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.062

KTGVLVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.04

KTKILVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.028

KTKLLVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.19

KTKVAVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.049

KTKVFVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.12

KTKVGVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.18

KTKVLFVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.12

KTKVLIVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.032 - 2

KTKVLVVQPK

6 entries

0.15

KTKVMVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.19

KTKVYVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.053

KTSVLVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

7.31 - 54.7

N6-(7-methoxycoumarin-4-yl)acetyl-L-Lys-L-Ala-L-Pro-L-Gln-L-Val-L-Leu-4-nitro-L-phenylalanine-L-Val-L-Met-L-His-L-Pro-L-Leu

2 entries

0.02

PPAILPIISE

Human T-cell leukemia virus type I

-

-

647862

0.014

PPVMGVLDAP

Human T-cell leukemia virus type I

-

-

647862

0.06

PQVLPVMHP

Human T-cell leukemia virus type I

-

-

647862

0.087

TKVLVVQPK

2 entries

0.26

VSQIYPIVQ

Human T-cell leukemia virus type I

-

pH 5.6, 37°C

647854

0.26

VSQLYPIVQ

Human T-cell leukemia virus type I

-

pH 5.6, 37°C

647854

0.42

VSQVYPIVQ

Human T-cell leukemia virus type I

-

pH 5.6, 37°C

647854

0.5

YVEPTAPQVLPVMHP

2 entries

additional information

additional information

3 entries

-

0.031

Ala-Pro-Gln-Val-Sta-Val-Met-His-Pro-Leu

Human T-cell leukemia virus type I

-

C-terminally truncated mutant of 116 residues, pH 5.6, 37°C

733121

0.032

Ala-Pro-Gln-Val-Sta-Val-Met-His-Pro-Leu

Human T-cell leukemia virus type I

-

C-terminally truncated mutant of 122 residues, pH 5.6, 37°C

733121

0.047

Ala-Pro-Gln-Val-Sta-Val-Met-His-Pro-Leu

Human T-cell leukemia virus type I

-

C-terminally truncated mutant of 121 residues, pH 5.6, 37°C

733121

0.042

APQVLF(NO2)VMHPL

Human T-cell leukemia virus type I

-

full length enzyme, 37°C, pH 5.6

717390

0.062

APQVLF(NO2)VMHPL

Human T-cell leukemia virus type I

-

truncated enzyme, residues 1-116, 37°C, pH 5.6

717390

0.098

APQVLPVMHP

Human T-cell leukemia virus type I

-

pH 5.6, 37°C, mutant enzyme L40I

647855

0.115

APQVLPVMHP

Human T-cell leukemia virus type I

-

pH 5.6, 37°C, wild-type enzyme

647855

0.38

APQVLPVMHP

Human T-cell leukemia virus type I

-

truncated enzyme, residues 1-116, 37°C, pH 5.6

717390

0.8

APQVLPVMHP

Human T-cell leukemia virus type I

-

full length enzyme, 37°C, pH 5.6

717390

0.3

APQVLPVMHPHG

Human T-cell leukemia virus type I

-

-

647858

0.47

APQVLPVMHPHG

Human T-cell leukemia virus type I

-

-

647851

0.47

APQVLPVMHPHG

Human T-cell leukemia virus type I

-

pH 5.3, 37°C

647849

0.0068

dabsyl-(GABA)-PQVLPVMH-EDANS

Human T-cell leukemia virus type I

-

pH 5.2, 37°C, modified L40I mutant enzyme lacking the 10 C-terminal amino acid residues

667390

0.01

dabsyl-(GABA)-PQVLPVMH-EDANS

Human T-cell leukemia virus type I

-

pH 5.2, 37°C, modified L40I wild-type enzyme

667390

0.23

DPASILPVIP

Human T-cell leukemia virus type I

-

-

647862

0.23

DPASILPVIP

Human T-cell leukemia virus type I

-

pH 5.6, 37°C, wild-type enzyme

647855

0.244

DPASILPVIP

Human T-cell leukemia virus type I

-

pH 5.6, 37°C, mutant enzyme L40I

647855

0.03

KGPPVILPIQAP

Human T-cell leukemia virus type I

-

pH 5.6, 37°C, wild-type enzyme

647855

0.034

KGPPVILPIQAP

Human T-cell leukemia virus type I

-

pH 5.6, 37°C, mutant enzyme L40I

647855

0.032

KTKVLVVQPK

Human T-cell leukemia virus type I

-

pH 5.6, 37°C, mutant enzyme L40I

647855

0.033

KTKVLVVQPK

Human T-cell leukemia virus type I

-

pH 5.6, 37°C, wild-type enzyme

647855

0.063

KTKVLVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.31

KTKVLVVQPK

Human T-cell leukemia virus type I

-

pH 5.3, 37°C

647851

0.52

KTKVLVVQPK

Human T-cell leukemia virus type I

-

full length enzyme, 37°C, pH 5.6

717390

1.5 - 2

KTKVLVVQPK

Human T-cell leukemia virus type I

-

truncated enzyme, residues 1-116, 37°C, pH 5.6

717390

7.31

N6-(7-methoxycoumarin-4-yl)acetyl-L-Lys-L-Ala-L-Pro-L-Gln-L-Val-L-Leu-4-nitro-L-phenylalanine-L-Val-L-Met-L-His-L-Pro-L-Leu

Human T-cell leukemia virus type I

-

mutant L40I, pH 5.3, 37°C

718025

54.7

N6-(7-methoxycoumarin-4-yl)acetyl-L-Lys-L-Ala-L-Pro-L-Gln-L-Val-L-Leu-4-nitro-L-phenylalanine-L-Val-L-Met-L-His-L-Pro-L-Leu

Human T-cell leukemia virus type I

-

His-tagged recombinant protein, pH 5.3, 37°C

718025

0.087

TKVLVVQPK

Human T-cell leukemia virus type I

-

-

647862

0.087

TKVLVVQPK

Human T-cell leukemia virus type I

-

5.6, 37°C

647854

0.5

YVEPTAPQVLPVMHP

Human T-cell leukemia virus type I

-

-

647862

0.5

YVEPTAPQVLPVMHP

Human T-cell leukemia virus type I

-

pH 5.0, 37°C

647849

additional information

additional information

Human T-cell leukemia virus type I

-

-

647862

-

additional information

additional information

Human T-cell leukemia virus type I

-

the Km-value of the gag protein is dependent on the concentration of (NH4)2SO4

647849

-

additional information

additional information

Human T-cell leukemia virus type I

-

kinetics of wild-type and mutant enzymes

669236

-