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3.4.23.B6: Mason-Pfizer monkey virus proteinase

This is an abbreviated version!
For detailed information about Mason-Pfizer monkey virus proteinase, go to the full flat file.

Word Map on EC 3.4.23.B6

Reaction

The enzyme cleaves 17 amino acids of the C-terminal 38-amino-acid cytoplasmic tail of the transmembrane protein TM of the released immature virus. =

Synonyms

M-PMV PR, M-PMV protease, Mason-Pfizer leukemia virus retropepsin, Mason-Pfizer monkey virus retropepsin, More, MPMV protease, P-MPV poteinase, P-MPV PR

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.B6 Mason-Pfizer monkey virus proteinase

Engineering

Engineering on EC 3.4.23.B6 - Mason-Pfizer monkey virus proteinase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A114R
-
site-directed mutagenesis, the mutation alters the substrate specificity and reduces the enzyme activity and viral infectivity of COS-1 cells
C7A/C106A
-
the midpoint of the urea unfolding curve for 12000 Da truncated enzyme is at 1.9 M urea, the denaturation curve for the C7A/C106A mutant is no longer sigmoidal in character, confirming that this mutant is very unstable
C7A/D26N/C106A
-
triple mutant of the 12000 Da truncated enzyme form shows significantly decreased capacity to dimerize
D26N/C7A/C106A
-
mutant of isozyme p12 showing altered structure and low target functions
H21A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
N109I/Q115I
-
site-directed mutagenesis, the mutation alters the substrate specificity and reduces the enzyme activity and viral infectivity of COS-1 cells
Q115I
-
site-directed mutagenesis, the mutation alters the substrate specificity and reduces the enzyme activity and viral infectivity of COS-1 cells
Q9A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, and mutant virions show reduced incorporation of the viral glycoprotein Env during virus assembly
Q9A/V20A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, and mutant virions show reduced incorporation of the viral glycoprotein Env during virus assembly
V20A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, and mutant virions show reduced incorporation of the viral glycoprotein Env during virus assembly
additional information
-
mutation of the cytoplasmic tail amino acids of the transmembrane protein TM, analysis of effects on the fusion to glycoprotein Env involving he protease activity, overview