3.4.23.B5: murine leukemia virus protease

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For detailed information about murine leukemia virus protease, go to the full flat file.

Reaction

processing of viral polyprotein. The retroviral protease is essential for virus replication, by processing of viral Gag and Gag-Pol polyproteins =

Synonyms

MLV PR, MLV protease, MLV retropepsin, MMLV protease, Mo-MuLV PR, Mo-MuLV protease, Moloney MLV retropepsin, Moloney murine leukemia virus protease, Moloney murine leukemia virus retropepsin, More, MuLV 4070A, MuLV proteinase, murine leukemia virus protease, viral protease, vPR

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.23 Aspartic endopeptidases

                3.4.23.B5 murine leukemia virus protease

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Results	in table
2	AA Sequence
1	Application
1	CAS Registry Number
9	Cloned(Commentary)
2	Crystallization (Commentary)
2	IC50 Value
19	Inhibitors
1	kcat/KM [mM/s]
12	Ki Value [mM]
94	KM Value [mM]
2	Metals/Ions
4	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
20	Organism
5	pH Optimum
1	pH Range
1	Posttranslational Modification
24	Protein Variants
5	Purification (Commentary)
3	Reaction
1	Reaction Type
20	Reference
2	Specific Activity [micromol/min/mg]
166	Substrates and Products (Substrate)
3	Subunits
19	Synonyms
4	Temperature Optimum [°C]
96	Turnover Number [1/s]

Purification

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Purification on EC 3.4.23.B5 - murine leukemia virus protease

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

by acetobne precipitation and extraction, overview, recombinnat enzyme with C-terminal GGSIEGR

Moloney murine leukemia virus

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669690

expression as His-tagged maltose-binding protein fusion enzyme from Escherichia coli, the fusion protein is cleaved off

Moloney murine leukemia virus

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666190

native enzyme partially, recombinant enzyme to homogeneity from Escherichia coli

Moloney murine leukemia virus

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668798

recombinant enzyme in fusion with the glutathione S-transferase from Schistosoma japonicum

Moloney murine leukemia virus

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647847

recombinant maltose-binding protein fusion enzyme from Escherichia coli strains DH5alpha and BL21, with or without His-tag, by amylose or nickel affinity chromatography, the fusion protein is cleaved of by factor Xa followed by cation exchange chromatography, non-His-tagged MBP-fusion-enzyme 2.6fold and His-tagged MBP-fusion enzyme 16.6fold to homogeneity, optimization of the purification method to diminish protein degradation, overview

murine leukemia virus

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670761