3.4.23.B20: HycD peptidase

This is an abbreviated version!
For detailed information about HycD peptidase, go to the full flat file.

Reaction

This enzyme specifically removes a 15-amino acid peptide from the C-terminus of the precursor of the large subunit of hydrogenase 2 [UniProt: P0ACE0] in Escherichia coli =

Synonyms

endopeptidase of the hydrogenase 2 operon, HYBD, hydrogenase maturating endopeptidase HYBD, HypD

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.23 Aspartic endopeptidases

                3.4.23.B20 HycD peptidase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
1	AA Sequence
1	Cloned(Commentary)
1	Crystallization (Commentary)
1	Metals/Ions
2	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
5	Organism
2	Purification (Commentary)
1	Reaction
5	Reference
6	Substrates and Products (Substrate)
1	Subunits
7	Synonyms

Substrates Products

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Substrates Products on EC 3.4.23.B20 - HycD peptidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Substrate/Product Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

precursor of the large subunit of hydrogenase 2 + H2O

?

2 entries

precursor of the large subunit of hydrogenase 2 + H2O

large subunit of hydrogenase 2 + propeptide of hydrogenase 2

2 entries

additional information

?

-

Escherichia coli

P37182

a construct in which the C-terminal extension from the large subunit of the hydrogenase 2 is fused to the mature part of the large subunit of hydrogenase 3 is neither processed by HybD nor by HycI (the endopeptidase specific for the large subunit of hydrogenase 3)

649503

-

-

?

precursor of the large subunit of hydrogenase 2 + H2O

?

Escherichia coli

P37182

-

651066, 690862

-

-

?

precursor of the large subunit of hydrogenase 2 + H2O

?

Escherichia coli

P37182

the final step of maturation of [NiFe]-hydrogenases involves the activity of an endopeptidase which removes an oligopeptide from the C-terminus of the large subunit of hydrogenase 2 (UniProt: P0ACE0). The proteolytic maturation is followed by a conformational change, closing of the metal centre, its assembly with the small hydrogenase subunit and finally in the appearance of enzyme activity

690862

-

-

?

precursor of the large subunit of hydrogenase 2 + H2O

large subunit of hydrogenase 2 + propeptide of hydrogenase 2

Escherichia coli

P37182

the maturation of [NiFe] hydrogenases includes formation of the nickel metallocenter, proteolytic processing of the metal center carrying large subunit, and its assembling with other hydrogenase subunits. The hydrogenase maturating enzyme HYBD specifically cleaves off a 15 amino acid peptide from the C terminus of the precursor of the large subunit of hydrogenase 2 (UniProt: P0ACE0)

693609

-

-

?

precursor of the large subunit of hydrogenase 2 + H2O

large subunit of hydrogenase 2 + propeptide of hydrogenase 2

Escherichia coli

P37182

specifically cleaves off a 15 amino acid peptide from the C terminus of the precursor of the large subunit of hydrogenase 2 (UniProt: P0ACE0)

693609

-

-

?