3.4.23.52: preflagellin peptidase
This is an abbreviated version!
For detailed information about preflagellin peptidase, go to the full flat file.
Word Map on EC 3.4.23.52
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3.4.23.52
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preflagellins
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methanococcus
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flagellins
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prepilins
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voltae
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flagellation
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flak
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maripaludis
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methanogen
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archaeon
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flagella
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pilins
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non-motile
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glass
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glycans
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sulfolobus
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iv-like
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unprocessed
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n-linked
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cotranscribed
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pilus-like
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non-flagellated
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preproteins
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12-amino-acid
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archaella
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synthesis
- 3.4.23.52
- preflagellins
- methanococcus
- flagellins
- prepilins
- voltae
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flagellation
-
flak
- maripaludis
-
methanogen
- archaeon
-
flagella
- pilins
-
non-motile
-
glass
- glycans
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sulfolobus
-
iv-like
-
unprocessed
-
n-linked
-
cotranscribed
-
pilus-like
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non-flagellated
- preproteins
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12-amino-acid
-
archaella
- synthesis
Reaction
Cleaves the signal peptide of 3 to 12 amino acids from the N-terminal of preflagellin, usually at Arg-Gly-/- or Lys-Gly-/-, to release flagellin. =
Synonyms
EppA, FlaK, peptidase-like enzyme, PibD, preflagellin peptidase, Prepilin peptidase, prepilin-like peptidase, RMVO00262, saci_0139, signal peptidase III, SPaseIII, SSO0131, type IV prepilin peptidase
ECTree
Advanced search results
Engineering
Engineering on EC 3.4.23.52 - preflagellin peptidase
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D186A
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the mutation does not affect the activity towards preflagellin FlaB2
D190A
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the mutation does not affect the activity towards preflagellin FlaB2
D224A
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the mutation does not affect the activity towards preflagellin FlaB2
D79E
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the mutant restores preflagellin peptidase activity (despite a reduced amount of peptidase produced)
D186A
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the mutation does not affect the activity towards preflagellin FlaB2
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D190A
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the mutation does not affect the activity towards preflagellin FlaB2
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D157A
the activity level of the mutant is comparable to that of the wild type enzyme
D173A
the activity level of the mutant is comparable to that of the wild type enzyme
D180A
the activity level of the mutant is comparable to that of the wild type enzyme
D207N
the activity level of the mutant is comparable to that of the wild type enzyme
D30A
the activity level of the mutant is comparable to that of the wild type enzyme
A77G
activity level is comparable to that of the wild type enzyme under standard assay conditions
A77P
D185A
activity of the mutant enzyme is lower than activity of wild-type enzyme
D186A
activity level is comparable to that of the wild type enzyme under standard assay conditions
I22E
activity level is comparable to that of the wild type enzyme under standard assay conditions
K23A
activity level is comparable to that of the wild type enzyme under standard assay conditions
K24A
activity level is comparable to that of the wild type enzyme under standard assay conditions
L81A
activity level is comparable to that of the wild type enzyme under standard assay conditions
R25A
single mutant displays Aap pili even though no preopillin AapA-processing is observed
D186A
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activity level is comparable to that of the wild type enzyme under standard assay conditions
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L81A
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activity level is comparable to that of the wild type enzyme under standard assay conditions
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R25A
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single mutant displays Aap pili even though no preopillin AapA-processing is observed
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additional information
activity level is comparable to that of the wild type enzyme under standard assay conditions
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construction of deletion mutant strains DELTAeppA, DELTAeppADELTAaglB, and DELTAflakDELTAeppA. AglB is the oligosaccharyltransferase responsible for the terminal step in the N-linked glycosylation pathway. In the DELTAeppA mutant where the signal peptide is not be removed from the pilins, the proteins EpdE and EpdD migrate faster than the same proteins in wild-type cells, despite the fact the proteins are larger due to the additional signal peptide in the DELTAeppA strain. In the DELTAaglBDELTAeppA double mutant, the electrophoretic mobility of EpdE-FLAG and EpdD-FLAG is the same as it was in the DELTAeppA strain
additional information
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construction of deletion mutant strains DELTAflak, DELTAflakDELTAaglB, and DELTAflakDELTAeppA. AglB is the oligosaccharyltransferase responsible for the terminal step in the N-linked glycosylation pathway
additional information
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construction of deletion mutant strains DELTAeppA, DELTAeppADELTAaglB, and DELTAflakDELTAeppA. AglB is the oligosaccharyltransferase responsible for the terminal step in the N-linked glycosylation pathway. In the DELTAeppA mutant where the signal peptide is not be removed from the pilins, the proteins EpdE and EpdD migrate faster than the same proteins in wild-type cells, despite the fact the proteins are larger due to the additional signal peptide in the DELTAeppA strain. In the DELTAaglBDELTAeppA double mutant, the electrophoretic mobility of EpdE-FLAG and EpdD-FLAG is the same as it was in the DELTAeppA strain
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additional information
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construction of deletion mutant strains DELTAflak, DELTAflakDELTAaglB, and DELTAflakDELTAeppA. AglB is the oligosaccharyltransferase responsible for the terminal step in the N-linked glycosylation pathway
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