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3.4.23.52: preflagellin peptidase

This is an abbreviated version!
For detailed information about preflagellin peptidase, go to the full flat file.

Word Map on EC 3.4.23.52

Reaction

Cleaves the signal peptide of 3 to 12 amino acids from the N-terminal of preflagellin, usually at Arg-Gly-/- or Lys-Gly-/-, to release flagellin. =

Synonyms

EppA, FlaK, peptidase-like enzyme, PibD, preflagellin peptidase, Prepilin peptidase, prepilin-like peptidase, RMVO00262, saci_0139, signal peptidase III, SPaseIII, SSO0131, type IV prepilin peptidase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.52 preflagellin peptidase

Engineering

Engineering on EC 3.4.23.52 - preflagellin peptidase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E25C/I206C
the mutant is proteolytically active
D186A
-
the mutation does not affect the activity towards preflagellin FlaB2
D18A
-
inactive
D18E
-
the mutation results in an apparently unstable protein
D18N
-
inactive
D190A
-
the mutation does not affect the activity towards preflagellin FlaB2
D224A
-
the mutation does not affect the activity towards preflagellin FlaB2
D79A
-
inactive
D79E
-
the mutant restores preflagellin peptidase activity (despite a reduced amount of peptidase produced)
D79N
-
inactive
D186A
-
the mutation does not affect the activity towards preflagellin FlaB2
-
D18A
-
inactive
-
D190A
-
the mutation does not affect the activity towards preflagellin FlaB2
-
D79A
-
inactive
-
D79N
-
inactive
-
D157A
the activity level of the mutant is comparable to that of the wild type enzyme
D173A
the activity level of the mutant is comparable to that of the wild type enzyme
D180A
the activity level of the mutant is comparable to that of the wild type enzyme
D187A
the mutant shows an apparent reduction in activity
D187A/D188A
the mutant does not cleave preflagellin
D188A
the mutant shows about wild type activity
D207N
the activity level of the mutant is comparable to that of the wild type enzyme
D23A
the mutant does not cleave preflagellin
D30A
the activity level of the mutant is comparable to that of the wild type enzyme
D80A
the mutant does not cleave preflagellin
A77E
mutant enzyme shows no processing of prepillin AapA
A77G
activity level is comparable to that of the wild type enzyme under standard assay conditions
D185A
activity of the mutant enzyme is lower than activity of wild-type enzyme
D186A
activity level is comparable to that of the wild type enzyme under standard assay conditions
D21A
mutant enzyme shows no processing of prepillin AapA
D78A
mutant enzyme shows no processing of prepillin AapA
G75E
mutant enzyme shows no processing of prepillin AapA
I22E
activity level is comparable to that of the wild type enzyme under standard assay conditions
K23A
activity level is comparable to that of the wild type enzyme under standard assay conditions
K24A
activity level is comparable to that of the wild type enzyme under standard assay conditions
L81A
activity level is comparable to that of the wild type enzyme under standard assay conditions
R25A
single mutant displays Aap pili even though no preopillin AapA-processing is observed
W32A
mutation results in reduced activity
D186A
-
activity level is comparable to that of the wild type enzyme under standard assay conditions
-
D21A
-
mutant enzyme shows no processing of prepillin AapA
-
D78A
-
mutant enzyme shows no processing of prepillin AapA
-
L81A
-
activity level is comparable to that of the wild type enzyme under standard assay conditions
-
R25A
-
single mutant displays Aap pili even though no preopillin AapA-processing is observed
-
additional information