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3.4.23.5: cathepsin D

This is an abbreviated version!
For detailed information about cathepsin D, go to the full flat file.

Word Map on EC 3.4.23.5

Reaction

Specificity similar to, but narrower than, that of pepsin A. Does not cleave the Gln4-His bond in B chain of insulin =

Synonyms

BmCatD, CAD 1, CAD 2, CAD 3, CapD, Cat D, CAT-D, CatD, Cath D, cath-D, cathD, cathepsin D, cathepsin D-like proteinase, cathepsin D1, cathepsin D2, CD1, CTSD, EC 3.4.4.23, matCTSD, PCD, Pep4p, preproCatD, pro-cathepsin, pro-cathepsin D, pro-CD, pro-CtsD, proCat, proCDrec, proCTSD, Proteinase A

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.5 cathepsin D

Temperature Stability

Temperature Stability on EC 3.4.23.5 - cathepsin D

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
-
10 min, at pH 3.5 or at pH 7.5, stable
20 - 65
-
completely stable between 20 and 40°C, thereafter activity decreases continuously, 20% loss of activity at 50°C, 80% loss of activity at 60°C, complete inactivation at 65°C
25
-
complete inactivation within 10 days
25 - 45
-
rapid decrease of activity below 25°C and above 45°C
25 - 60
-
the protease is heat stable at ambient pressure up to 300 MPa and 55°C, respectively, causing less than 10% inactivation after 10 min treatment. Cathepsin D is quickly inactivated at temperatures above 60°C
4
-
72 h, complete loss of activity
43
-
10 min, stable at pH 3.5, 40% loss of activity at pH 7.5
45
-
30 min, 30% loss of activity
45 - 55
-
the protease begins to thermally unfold above 45°C, with a melting temperature of 53°C. The enzyme is inactivated at 55°C
46
-
heat inactivation proceeds linearly with time for the control enzyme but biphasically for the enzyme from animals with experimental neurofibrillary changes
48.5
-
10 min, stable at pH 3.5, 81% loss of activity at pH 7.5
5 - 45
-
the enzyme is highly stable at temperatures below 45°C but activity declines rapidly at temperatures exceeding 50°C no activity is detected after incubation at 65°C for 60 min
50 - 60
-
inactivation at the temperature of 50-60°C
54
-
10 min, 13% loss of activity at pH 3.5, complete loss of activity at pH 7.5
57
-
pH 3, 90 min, 94% loss of activity
65
-
30 min, complete inactivation
66
-
10 min, complete loss of activity at pH 3.5 and at pH 7.5
80
-
the enzyme retains up to 4% alpha helix at 80°C