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3.4.23.5: cathepsin D

This is an abbreviated version!
For detailed information about cathepsin D, go to the full flat file.

Word Map on EC 3.4.23.5

Reaction

Specificity similar to, but narrower than, that of pepsin A. Does not cleave the Gln4-His bond in B chain of insulin =

Synonyms

BmCatD, CAD 1, CAD 2, CAD 3, CapD, Cat D, CAT-D, CatD, Cath D, cath-D, cathD, cathepsin D, cathepsin D-like proteinase, cathepsin D1, cathepsin D2, CD1, CTSD, EC 3.4.4.23, matCTSD, PCD, Pep4p, preproCatD, pro-cathepsin, pro-cathepsin D, pro-CD, pro-CtsD, proCat, proCDrec, proCTSD, Proteinase A

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.5 cathepsin D

Cloned

Cloned on EC 3.4.23.5 - cathepsin D

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a vaccinia virus expression system is used for the production of recombinant human cathepsin D in human cell lines MCF7 (breast cancer), HepG2 (hepatocarcinoma) and HeLa (cervix cancer) and non-human cell line BSC1 (simian kidney epithelial). For generation of a recombinant vaccinia virus the full-length cathepsin D cDNA comprising the ATG codon is inserted downstream of a synthetic vaccinia late promoter into the vector pMJ601 which also contains the Escherichia coli LacZ gene. This expression cassette is flanked by segments of the vaccinia thymidine kinase (TK) gene that allows homologous recombination with the wild-type virus
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baculovirus expression vector system, using the Autographa californica nucleopolyhedrovirus (AcNPV) and insect cell line Sf9, is employed for the production of recombinant BmCatD protein
CAD 3 is expressed in Escherichia coli strain Origami DE3
expressed in Drosophila S2 cells
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expressed in Escherichia coli
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expressed in Escherichia coli BL21(DE3) cells
expressed in HEK-293 cells
expressed in HEK-293FT cells
-
expressed in Sf9 insect cells
expressed in SH-SY5Y cells and PAC-2 cells
the propeptide is expressed in Escherichia coli BL21(DE3) cells