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3.4.23.49: omptin

This is an abbreviated version!
For detailed information about omptin, go to the full flat file.

Word Map on EC 3.4.23.49

Reaction

Has a virtual requirement for Arg in the P1 position and a slightly less stringent preference for this residue in the P1' position, which can also contain Lys, Gly or Val. =

Synonyms

bacterial outer-membrane protease, Citrobacter rodentium outer-membrane protease, CroP, E. coli protease VII, EC 3.4.21.87, endoprotease, Gene ompT proteins, More, OmpP, OmpP protease, ompT, OmpT protease, OmpT protein, Omptin, omptin protease, outer membrane protease, Outer membrane protein 3B, outer-membrane protease, outer-membrane protease T, PgtE, Pla, plaA, protease 7, Protease A, Protease VII, Protein a, Proteins, specific or class, gene ompT, SopA

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.49 omptin

Engineering

Engineering on EC 3.4.23.49 - omptin

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D210A
-
site-directed mutagenesis by overlap extension PCR
H212A
-
site-directed mutagenesis by overlap extension PCR
D208A
-
introduced as silent mutation in plasmids, transformation with plasmids
D208G
-
site-directed mutagenesis, the mutant enzyme shows increased specificity for the A-R cleavage site compared to the wild-type enzyme
D210A
-
introduced as silent mutation in plasmids, transformation with plasmids
D43A
-
introduced as silent mutation in plasmids, transformation with plasmids
D83A
-
introduced as silent mutation in plasmids, transformation with plasmids
D85A
-
introduced as silent mutation in plasmids, transformation with plasmids
D97C
-
site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, substrate specificity with fusion protein, overview
D97F
-
site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, substrate specificity with fusion protein, overview
D97H
-
site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, preference for human calcitonin precursor, substrate specificity with fusion protein, overview
D97L
-
site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, preference for human adrenocarticotropic hormone, substrate specificity with fusion protein, overview
D97M
-
site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, preference for a fusion peptide substrate with the sequence R-R-R-A-R*-motilin, substrate specificity with fusion protein, overview
D97N
-
site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, substrate specificity with fusion protein, overview
D97Q
-
site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, substrate specificity with fusion protein, overview
D97S
-
site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, substrate specificity with fusion protein, overview
D97T
-
site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, substrate specificity with fusion protein, overview
E111A
-
introduced as silent mutation in plasmids, transformation with plasmids
E136A
-
introduced as silent mutation in plasmids, transformation with plasmids
E193A
-
introduced as silent mutation in plasmids, transformation with plasmids
E250A
-
introduced as silent mutation in plasmids, transformation with plasmids
E27A
-
introduced as silent mutation in plasmids, transformation with plasmids
G216K/K217G
H212A
molecular mechanics/coarse-grained (MM/CG) simulation applied
S223R
-
site-directed mutagenesis, the mutant enzyme shows increased specificity for the A-R cleavage site and overall reduced activity compared to the wild-type enzyme
S99A
molecular mechanics/coarse-grained (MM/CG) simulation applied
S99A/G216K/K217G
-
recombinant ompT variant in order to abolish autoproteolysis
D206A
additional information