Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.23.48: plasminogen activator Pla

This is an abbreviated version!
For detailed information about plasminogen activator Pla, go to the full flat file.

Word Map on EC 3.4.23.48

Reaction

Converts human Glu-plasminogen to plasmin by cleaving the Arg560-/-Val peptide bond that is also hydrolysed by the mammalian u-plasminogen activator and t-plasminogen activator. Also cleaves arginyl bonds in other proteins =

Synonyms

beta-Pla, coagulase, fibrinolysin, outer membrane protease, Pla, Pla protease, plasminogen activator, plasminogen activator Pla, plasminogen activator protease Pla, YapE

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.48 plasminogen activator Pla

Cloned

Cloned on EC 3.4.23.48 - plasminogen activator Pla

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
BL21-DE3 used as a host to express recombinant protein, cloning into pBluescript SK+ to produce plasmid pBLA4L
expressed in Escherichia coli TG1, strains BL21 and BL21(DE3)pLys used for expression of recombinant proteins
-
expression in Escherichia coli
expression in Escherichia coli and Yersinia pseudotuberculosis
-
expression of 9.5 kb plasmid harboring the Pla gene in Escherichia coli
-
gene pla, recombinant expression in a DELTAcroP mutant strain of Citrobacter rodentium and in Escherichia coli strain BL21
Pla is encoded by the 9.5 kb Yersinia pestis plasmid pPCP, sequence comparisons
Pla is encoded by the 9.5 kb Yersinia pestis plasmid pPCP, sequencing of pla genes from 118 Yersinia pestis subsp. microtus isolates, sequence comparisons
tetracycline-responsive promoter system in Yersinia pestis applied to control primary pneumonic plague progression after activation/inhibition of plasminogen activator Pla expression
the mutant enzyme T259I is expressed in Escherichia coli XL1 Blue cells and in Yersinia pestis strain KIM D34
-