3.4.23.41: yapsin 1

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For detailed information about yapsin 1, go to the full flat file.

Word Map on EC 3.4.23.41

3.4.23.41

prohormone

granules

monobasic

propeptide

aspartyl

pituitary

cholecystokinin

non-anchored

tetrabasic

parathyroid

pepstatin

endoglycosidase

acth

zymogen

pheromone

dibasic

pro-opiomelanocortin

proproteins

residue-specific

prohormone-processing

synthesis

medicine

Reaction

hydrolyses various precursor proteins with Arg or Lys in P1, and commonly Arg or Lys also in P2. The P3 amino acid is usually non-polar, but otherwise additional basic amino acids are favourable in both non-prime and prime positions =

Synonyms

aspartic protease yapsin I, aspartic proteinase 3, Kex2, More, opsA, Yap3 gene product, yeast aspartic protease, yeast aspartic protease 3, YPS1, YPS7

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.23 Aspartic endopeptidases

3.4.23.41 yapsin 1

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Results	in table
11	AA Sequence
3	Application
1	CAS Registry Number
10	Cloned(Commentary)
2	Expression
13	General Information
10	Inhibitors
3	Ki Value [mM]
6	KM Value [mM]
6	Localization
2	Metals/Ions
11	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
106	Organism
4	pH Optimum
1	pH Range
1	pI Value
6	Posttranslational Modification
6	Protein Variants
7	Purification (Commentary)
4	Reaction
1	Reaction Type
30	Reference
1	Specific Activity [micromol/min/mg]
36	Substrates and Products (Substrate)
7	Subunits
23	Synonyms
2	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]

Purification

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Purification on EC 3.4.23.41 - yapsin 1

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2 entries

recombinant enzyme lacking the GPI-anchor from yeast, purification using inhibitor RVSMIKNR affinity chromatography

Saccharomyces cerevisiae

668790

recombinant truncated enzyme

Ogataea angusta

Q3L7F4, Q5QT58

718457

truncated proyapsin I expressed in baculovirus/Sf9 cells

Saccharomyces cerevisiae

36898

truncated yapsin I expressed in yeast

Saccharomyces cerevisiae

36898

using Ni-NTA chromatography

Aspergillus oryzae

Q8NKB5

707789