3.4.23.41: yapsin 1

This is an abbreviated version!
For detailed information about yapsin 1, go to the full flat file.

Reaction

hydrolyses various precursor proteins with Arg or Lys in P1, and commonly Arg or Lys also in P2. The P3 amino acid is usually non-polar, but otherwise additional basic amino acids are favourable in both non-prime and prime positions =

Synonyms

aspartic protease yapsin I, aspartic proteinase 3, Kex2, More, opsA, Yap3 gene product, yeast aspartic protease, yeast aspartic protease 3, YPS1, YPS7

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.23 Aspartic endopeptidases

                3.4.23.41 yapsin 1

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Results	in table
11	AA Sequence
3	Application
1	CAS Registry Number
10	Cloned(Commentary)
2	Expression
13	General Information
10	Inhibitors
3	Ki Value [mM]
6	KM Value [mM]
6	Localization
2	Metals/Ions
11	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
106	Organism
4	pH Optimum
1	pH Range
1	pI Value
6	Posttranslational Modification
6	Protein Variants
7	Purification (Commentary)
4	Reaction
1	Reaction Type
30	Reference
1	Specific Activity [micromol/min/mg]
36	Substrates and Products (Substrate)
7	Subunits
23	Synonyms
2	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]

General Information

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General Information on EC 3.4.23.41 - yapsin 1

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

malfunction

[Candida] glabrata

-

an enzyme-deficient mutant displays deregulated pH homeostasis

754781

metabolism

2 entries

physiological function

10 entries

metabolism

Saccharomyces cerevisiae

P32329

replacement of the N-entrance loop of yapsin Yps1 generates a single chain endopeptidase that undergoes at pH 6.0 partial or at pH 3.0 complete pro-peptide removal. At both pH, the shedding activity of the chimeric endopeptidase, where the N-entrance loop is replaced by the pentapeptide GSVMD found in Yps3, toward Gas1 and itself is strongly and drastically increased, respectively. A direct correlation between endoproteolytic cleavage of this loop in native Yps1 and its shedding is observed. The Yps1-dependent shedding of model glycosylphosphatidylinositol proteins Gas1 and Yps1 is also stimulated by the absence of the O-mannosyltransferases, Pmt4 and Pmt2 respectively. Under these conditions, some Yps1-independent shedding is also observed

733582

metabolism

Saccharomyces cerevisiae ATCC 204508

-

replacement of the N-entrance loop of yapsin Yps1 generates a single chain endopeptidase that undergoes at pH 6.0 partial or at pH 3.0 complete pro-peptide removal. At both pH, the shedding activity of the chimeric endopeptidase, where the N-entrance loop is replaced by the pentapeptide GSVMD found in Yps3, toward Gas1 and itself is strongly and drastically increased, respectively. A direct correlation between endoproteolytic cleavage of this loop in native Yps1 and its shedding is observed. The Yps1-dependent shedding of model glycosylphosphatidylinositol proteins Gas1 and Yps1 is also stimulated by the absence of the O-mannosyltransferases, Pmt4 and Pmt2 respectively. Under these conditions, some Yps1-independent shedding is also observed

-

physiological function

Aspergillus oryzae

Q8NKB5

complementation analysis of opsA and opsB in kexB mutants: overexpression of opsA or ops B (aspartyl proteases homologous to yeast yapsins YPS1/2) do not suppress the kexB mutation in Aspergillus oryzae or Aspergillus nidulans, although yapsins are multicopy suppressors for the yeast kex2 mutation

707789

physiological function

Ogataea angusta

Q3L7F4, Q5QT58

deletion of the YPS genes generates only minor influence on the sensitivity to cell wall stress

718457

physiological function

Ogataea angusta

Q3L7F4, Q5QT58

deletion of the YPS genes generates only minor influence on the sensitivity to cell wall stress. The sole disruption of YPS1 is sufficient in eliminating the aberrant proteolytic cleavage of recombinant proteins secreted by Hansenula polymorpha

718457

physiological function

[Candida] glabrata

-

Yps1 is required for cell growth at elevated temperatures

717611

physiological function

[Candida] glabrata

-

a Candida glabrata mutant lacking all 11 yapsins, possesses an enlarged vacuole and displays phenotypes similar to deficiency in vacuolar membrane ATPase with elevated metal ion susceptibility in an alkaline pH medium and diminished in vacuolar membrane ATPase activity. the mutant shows elevated polyphosphate levels and diminished cellular carboxypeptidase Y activity. A gross perturbation of cellular homoeostasis in the mutant lacking all yapsins, even in the absence of external stressors, is characterized by reduced levels of ATP and stress metabolites, elevated reactive oxygen species levels, cell surface abnormalities, and a constitutively activated protein kinase C signalling pathway. Isoform Yps1 is involved in the maintenance of ion homoeostasis under normal and calcineurin-inhibited conditions

733295

physiological function

Komagataella pastoris

-

disruption of yapsin YPS7 gene confers the mutant cell increased resistance to cell wall perturbing reagents congo red, calcofluor white and sodium dodecyl sulfate. Mutant shows lower content of chitin and increased amounts of beta-1,3-glucan. Disruption of YPS7 gene causes a reduction of the chitin content in lateral cell wall. The inner layer of mutant cell wall, mainly composed of chitin and beta-1, 3-glucan, is much thicker than that in parental strain GS115. The mutant also exhibits increased osmotic resistance compared with the parental strain

733889

physiological function

[Candida] glabrata

A0A1S7BH59

the enzyme is a potential virulence factor and involved in maturation of proteins and cell wall function

753595

physiological function

[Candida] glabrata

-

the enzyme is required for cell wall maintenance, remodeling and cell-cell interactions. The enzyme is essential for survival in an acidic environment

754781

physiological function

Komagataella pastoris GS115

-

disruption of yapsin YPS7 gene confers the mutant cell increased resistance to cell wall perturbing reagents congo red, calcofluor white and sodium dodecyl sulfate. Mutant shows lower content of chitin and increased amounts of beta-1,3-glucan. Disruption of YPS7 gene causes a reduction of the chitin content in lateral cell wall. The inner layer of mutant cell wall, mainly composed of chitin and beta-1, 3-glucan, is much thicker than that in parental strain GS115. The mutant also exhibits increased osmotic resistance compared with the parental strain

-

physiological function

[Candida] glabrata CBS138

-

the enzyme is a potential virulence factor and involved in maturation of proteins and cell wall function

-