3.4.23.40: Phytepsin

This is an abbreviated version, for detailed information about Phytepsin, go to the full flat file.

Reaction

Prefers hydrophobic residues Phe, Val, Ile, Leu, and Ala at P1 and P1', but also cleaves -Phe-/-Asp- and -Asp-/-Asp- bonds in 2S albumin from plant seeds =

Synonyms

Acid protease, Ap1, Aspartic proteinase, Aspartyl endoproteinase, Barley grain aspartic proteinase, Carboxyl proteinase, CardA, cardB, Cardosin, cardosin A, cardosin B, Cynarase, cynarase A, cynarase B, cynarase C, Edestinase, HvAP, Nepenthesin, Oryzasin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.40 Phytepsin

Molecular Weight

Molecular Weight on EC 3.4.23.40 - Phytepsin

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9000
-
1 * 29000 + 1 * 9000, SDS-PAGE
11500
-
1 * 29700 + 1 * 13300, cynarase A, 1 * 34500 + 1 * 11500, cynarase B, 1 * 32300 + 1 * 13300, cynarase C, SDS-PAGE
13300
-
1 * 29700 + 1 * 13300, cynarase A, 1 * 34500 + 1 * 11500, cynarase B, 1 * 32300 + 1 * 13300, cynarase C, SDS-PAGE
15000
x * 31000, and x * 15000, SDS-PAGE; x * 34000, and x * 15000, SDS-PAGE
20000
-
ultracentirfugation
26000
-
x * 47000, and x * 37000, processing intermediates, x * 26000 + x * 11000, associated by disulfide bridge, mature enzyme, SDS-PAGE
28000
-
gel filtration
29700
-
1 * 29700 + 1 * 13300, cynarase A, 1 * 34500 + 1 * 11500, cynarase B, 1 * 32300 + 1 * 13300, cynarase C, SDS-PAGE
31000
x * 31000, and x * 15000, SDS-PAGE
32000
-
1 * 32000 + 1 * 16000 (48-kDa aspartic proteinase, precursor of the 40-k-Da aspartic proteinase), 1 * 29000 + 1 * 11000 (40-k-Da aspartic proteinase), SDS-PAGE
32300
-
1 * 29700 + 1 * 13300, cynarase A, 1 * 34500 + 1 * 11500, cynarase B, 1 * 32300 + 1 * 13300, cynarase C, SDS-PAGE
34500
-
1 * 29700 + 1 * 13300, cynarase A, 1 * 34500 + 1 * 11500, cynarase B, 1 * 32300 + 1 * 13300, cynarase C, SDS-PAGE
36000 - 37000
-
ultracentrifugation, gel filtration
36000 - 40000
-
gel filtration
39000
-
PAGE
40000 - 42000
40000
-
2 * 40000, sequence of cDNA
40800
-
gel filtration, cynarase A
43400
-
gel filtration, cynarase B
46000
-
gel filtration, cynarase C
47000
-
x * 47000, and x * 37000, processing intermediates, x * 26000 + x * 11000, associated by disulfide bridge, mature enzyme, SDS-PAGE
50000
-
seeds, gel filtration
52600
x * 37000, SDS-PAGE, mature enzyme. x * 52600, calculated for proenzyme
55230
x * 55230, calculated
58000
-
1 * 58000, SDS-PAGE
59000
-
gel filtration
60000 - 65000
-
gel filtration
60500
-
gel filtration
66000
Triticum durum x Haynaldia villosa
-
gel filtration
66500
-
1 * 66500, SDS-PAGE
89000
-
leaf, gel filtration