3.4.23.40: Phytepsin

This is an abbreviated version, for detailed information about Phytepsin, go to the full flat file.

Reaction

Prefers hydrophobic residues Phe, Val, Ile, Leu, and Ala at P1 and P1', but also cleaves -Phe-/-Asp- and -Asp-/-Asp- bonds in 2S albumin from plant seeds =

Synonyms

Acid protease, Ap1, Aspartic proteinase, Aspartyl endoproteinase, Barley grain aspartic proteinase, Carboxyl proteinase, CardA, cardB, Cardosin, cardosin A, cardosin B, Cynarase, cynarase A, cynarase B, cynarase C, Edestinase, HvAP, Nepenthesin, Oryzasin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.40 Phytepsin

Expression

Expression on EC 3.4.23.40 - Phytepsin

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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene expression under postharvest chilling treatment in two pineapple varieties differing in their resistance to blackheart development reveals opposite trends. The resistant variety shows an up-regulation of AP1 precursor gene expression whereas the susceptible shows a down-regulation in response to postharvest chilling treatment. The same trend is observed regarding specific aspartic protease enzyme activity in both varieties
optimization of culture conditions for expression in Kluyveromyces lactis, best conditions are YPGal 4% for 120 h at 30°C. Subjecting the precursor form to a protein engineering approach followed by codon optimization leads to a clear improvement in the production of the protease, which also results in a reduction in expression time