3.4.23.40: Phytepsin

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For detailed information about Phytepsin, go to the full flat file.

Reaction

Prefers hydrophobic residues Phe, Val, Ile, Leu, and Ala at P1 and P1', but also cleaves -Phe-/-Asp- and -Asp-/-Asp- bonds in 2S albumin from plant seeds =

Synonyms

Acid protease, Ap1, Aspartic proteinase, Aspartyl endoproteinase, Barley grain aspartic proteinase, Carboxyl proteinase, CardA, cardB, Cardosin, cardosin A, cardosin B, Cynarase, cynarase A, cynarase B, cynarase C, Edestinase, HvAP, Nepenthesin, Oryzasin, PCB, procardosin B

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.23 Aspartic endopeptidases

                3.4.23.40 Phytepsin

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Results	in table
63	AA Sequence
3	Application
1	CAS Registry Number
4	Cloned(Commentary)
2	Crystallization (Commentary)
2	Expression
2	General Information
15	Inhibitors
7	kcat/KM [mM/s]
4	Ki Value [mM]
10	KM Value [mM]
2	Localization
48	Molecular Weight [Da]
19	Natural Substrates/ Products (Substrates)
43	Organism
3	PDB ID
31	pH Optimum
6	pH Range
1	pH Stability
4	pI Value
8	Posttranslational Modification
1	Protein Variants
5	Purification (Commentary)
1	Reaction
1	Reaction Type
43	Reference
39	Source Tissue
2	Specific Activity [micromol/min/mg]
2	Storage Stability
68	Substrates and Products (Substrate)
16	Subunits
24	Synonyms
5	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Temperature Stability [°C]
9	Turnover Number [1/s]

Expression

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Expression on EC 3.4.23.40 - Phytepsin

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EXPRESSION

ORGANISM

UNIPROT

LITERATURE

gene expression under postharvest chilling treatment in two pineapple varieties differing in their resistance to blackheart development reveals opposite trends. The resistant variety shows an up-regulation of AP1 precursor gene expression whereas the susceptible shows a down-regulation in response to postharvest chilling treatment. The same trend is observed regarding specific aspartic protease enzyme activity in both varieties

Ananas comosus

K7P8F2

734555

optimization of culture conditions for expression in Kluyveromyces lactis, best conditions are YPGal 4% for 120 h at 30°C. Subjecting the precursor form to a protein engineering approach followed by codon optimization leads to a clear improvement in the production of the protease, which also results in a reduction in expression time

Cynara cardunculus

Q9XFX4

733194