Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.23.4: chymosin

This is an abbreviated version!
For detailed information about chymosin, go to the full flat file.

Word Map on EC 3.4.23.4

Reaction

Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single Ser-Phe105-/-Met-Ala bond in kappa-chain of casein =

Synonyms

CHY-MAX, CHY-MAX 200, chymase, chymosin, chymosin A, chymosin B, CYM, EC 3.4.4.3, More, Preprorennin, prochymosin, rCH, rennin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.4 chymosin

pH Stability

pH Stability on EC 3.4.23.4 - chymosin

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2
-
-
36882
2 - 3.8
-
-
667435
2 - 6
-
under incubation at 37°C for 24 and 48 h, loss of activity at pH 3.5 and pH 7.0, trangenic chymosin and recombinant chymosin are inactivated slightly faster than the native enzyme
667763
2 - 6.5
-
crystalline chymosin exhibits optimum stability at pH between 5.3 and 6.3, the enzyme is moderately stable at pH 2.0, but the enzyme is unstable at pH around 3.5 and above 6.5, clotting activity is not observed at pH 7.0
708236
2 - 8
-
the enzyme retains 80% of activity within pH 2.0-8.0
731169
2.5 - 6.5
the recombinant enzyme is highly active and stable over a wide pH range (from 2.5 to 6.0) at 20°C for 8 h. Relative clotting activity declines when the recombinant chymosin is kept at a pH value above 6.0. No detectable clotting activity at pH 6.5
732866
3.5
-
rapid loss of activity
36882
3.5 - 7
the enzyme remains stable (100% activity) for 1 h at pH 3.5-7.0. At pH 7.5 and 8.0, the activity drops to about 40% and 20%, respectively. The enzyme is inactive after 1 h at pH 9.0
755314
5.3 - 6.3
-
highest stability
36882
7
-
rapid loss of activity above
36882