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3.4.23.4: chymosin

This is an abbreviated version!
For detailed information about chymosin, go to the full flat file.

Word Map on EC 3.4.23.4

Reaction

Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single Ser-Phe105-/-Met-Ala bond in kappa-chain of casein =

Synonyms

CHY-MAX, CHY-MAX 200, chymase, chymosin, chymosin A, chymosin B, CYM, EC 3.4.4.3, More, Preprorennin, prochymosin, rCH, rennin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.4 chymosin

pH Range

pH Range on EC 3.4.23.4 - chymosin

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1 - 5
-
acid-denatured hemoglobin, pH 1: about 80% of maximum activity, pH 5: about 30% of maximum activity
2 - 6
-
-
2 - 6.7
3 - 10
-
pH 3: about 80% of maximum activity, pH 10: about 20% of maximum activity
4 - 8
the enzyme shows about 60% activity at pH 4.0, about 80% activity at pH 5.5, 100% activity at pH 6.0, about 95% activity at pH 7.0, about 38% activity at pH 7.5, and about 20% activity at pH 8.0
4.5
-
using alpha- and beta-casein as substrate
4.6 - 5.6
-
-
4.85
-
calculated isoelectric point
5 - 6.5
the enzyme retains about 65%, 100%, 85% and 69% of milk clotting activity at pH 5.0, 5.5, 6.0 and 6.5, respectively
5 - 6.9
-
recombinant chymosin shows coagulant activity from pH 5.0 to 6.9
5 - 9
-
the Tol-A-III-chymosin fusion protein shows more than 60% activity between pH 5.0 and 9.0
5.1 - 6.7
at pH 5.1, the relative clotting activity decreases to 50% and further declines to 17% and 10% at pH 5.6 and pH 6.7, respectively
5.5 - 6
5.5 - 8
sharp decline of activity up to pH 5.8, moderate decline at higher pH with complete loss of activity at pH 8
5.6
-
-
5.8
-
using whole casein as substrate