3.4.23.38: plasmepsin I

This is an abbreviated version!
For detailed information about plasmepsin I, go to the full flat file.

Reaction

hydrolysis of the -Phe33-/-Leu- bond in the alpha-chain of hemoglobin, leading to denaturation of the molecule =

Synonyms

AH I, aspartic hemoglobinase I, malaria aspartic hemoglobinase, PFAPG, PfPM1, plasmepsin 1, plasmepsin I, plasmepsin-I, PLm I, PM I, PM-I, PMI

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.23 Aspartic endopeptidases

                3.4.23.38 plasmepsin I

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Results	in table
3	AA Sequence
1	CAS Registry Number
7	Cloned(Commentary)
1	Crystallization (Commentary)
1	General Information
44	IC50 Value
201	Inhibitors
139	Ki Value [mM]
18	KM Value [mM]
7	Localization
2	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
33	Organism
1	Pathway
6	PDB ID
8	pH Optimum
3	pH Range
5	Posttranslational Modification
1	Protein Variants
5	Purification (Commentary)
1	Reaction
1	Reaction Type
33	Reference
5	Source Tissue
1	Specific Activity [micromol/min/mg]
38	Substrates and Products (Substrate)
2	Subunits
20	Synonyms
17	Turnover Number [1/s]

Cloned

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Cloned on EC 3.4.23.38 - plasmepsin I

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Plasmodium falciparum

-

648179

expression and partial characterization of soluble recombinant PM I from Plasmodium falciparum in which a truncated form of PM I (Lys77P-Leu329) (P indicates a propart residues) is fused to thioredoxin in the pET32b(1) vector, Trx-tPM I and expressed in Escherichia coli Rosetta-gami B (DE3)pLysS. The soluble fusion protein is purified from cell culture using a combination of Ni21 affinity and gel filtration chromatography and is capable of autocatalytic activation at pH 4.0–5.5, which occurrs at Leu116P-Ser117P, seven residues upstream of the native cleavage site (Gly123P-Asn1)

Plasmodium falciparum

-

682637

expression in Escherichia coli

2 entries

expression of proplasmepsin I and V110P-proplasmepsin in Escherichia coli

Plasmodium falciparum

-

648190

expression of truncated proPfPM1 K110pN mutant in Escherichia coli

Plasmodium falciparum

P39898

696329

initial attempts to express full-length proplasmepsin I gene in Escherichia coli results in very low expression levels, possibly due to the toxic effects of the hydrophobic segment, and no activation to mature plasmepsin I is ever observed. Recombinant proplasmepsin I is expressed from pETPMI, a construct that is prepared from pET3a and contains the last 48 residues of the propart followed by the 328 residues of mature plasmepsin I. It is necessary to introduce a processing site, V110P, into the zymogen that renders the precursor capable of undergoing autoactivation

Plasmodium falciparum

-

648190

expression in Escherichia coli

Plasmodium falciparum

-

648189

expression in Escherichia coli

Plasmodium falciparum

P39898

718040