3.4.23.34: cathepsin E
This is an abbreviated version!
For detailed information about cathepsin E, go to the full flat file.
Word Map on EC 3.4.23.34
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3.4.23.34
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cathepsins
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proteinases
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pepstatin
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pepsinogen
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procathepsin
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molecular biology
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gastricsin
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medicine
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foveolar
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non-lysosomal
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3.4.23.5
- 3.4.23.34
- cathepsins
- proteinases
- pepstatin
- pepsinogen
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procathepsin
- molecular biology
- gastricsin
- medicine
-
foveolar
-
non-lysosomal
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3.4.23.5
Reaction
similar to cathepsin D, but slightly broader specificity =
Synonyms
CatE, Cathepsin D-like acid proteinase, cathepsin D-like aspartic proteinase, cathepsin D-like protease, Cathepsin D-type proteinase, cathepsin E, Cathepsin E-like acid proteinase, CE, CTSE, EMAP, erythrocyte membrane acid proteinase, Erythrocyte membrane aspartic proteinase, gastric mucosa non-pepsin acid proteinase, More, Non-pepsin proteinase, slow moving proteinase, Slow-moving proteinase, SMP
ECTree
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Cloned
Cloned on EC 3.4.23.34 - cathepsin E
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construction of two fusion proteins using chimeric DNAs encoding the cathepsin E propeptide fused to the mature cathepsin D tagged with HA at the COOH terminus and encoding the cathepsin D propeptide fused to the mature cathepsin E
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guinea pig
HEK-293 cells are transfected with the full length human cathepsin E gene cloned into pcDNA3.1V5His
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human
human pro-cathepsin E is expressed in Escherichia coli in the form of inclusion bodies. The protein is dissolved in 8 M guanidinium chloride and refolded by dilution/dialysis. The main side products in the refolding reaction were soluble, high molecular mass protein complexes linked most likely due to formation of wrong intra- and intermolecular disulfide bonds. Pro-cathepsin E auto-activates at pH 3.5. The major part of the high molecular mass complexes is easily removed during the auto-activation process as these protein components precipitate during the pH shifts
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mutants with changed active-site residues and lacking propeptides and N-glycosylation, expressed in human embryonic kidney 293T cells
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procathepsin E
rabbit
stable expression in human prostate carcinoma cell line ALVA101, product is named ALVA101/hCE
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