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3.4.23.30: Pycnoporopepsin

This is an abbreviated version!
For detailed information about Pycnoporopepsin, go to the full flat file.

Word Map on EC 3.4.23.30

Reaction

Similar to pepsin A, but narrower, cleaving only three bonds in the B chain of insulin: Ala14-/-Leu, Tyr16-/-Leu, and Phe24-/-Phe =

Synonyms

carboxyl proteinase I, EC 3.4.23.6, EC 3.4.4.17, EC 3.4.99.25, Proteinase Ia, Proteinase, Pycnoporus coccineus aspartic, Proteinase, Trametes acid, Trametes acid proteinase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.30 Pycnoporopepsin

Purification

Purification on EC 3.4.23.30 - Pycnoporopepsin

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
i.e. Pycnoporus coccineus, Trametes sanguinea
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native isozymes Ia and Ib 30fold to homogeneity from mycel-free culture filtrate, by acetone precipitation, anion exchange chromatograph, ammonium sulfate fractionation, dialysis and crystallization to homogeneity, and further to puritiy by anion exchange and sulfopropyl chromatograpgy, and isoelectric focusing
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