3.4.23.25: saccharopepsin

This is an abbreviated version!
For detailed information about saccharopepsin, go to the full flat file.

Reaction

Hydrolysis of proteins with broad specificity for peptide bonds. Cleaves -Leu-Leu-/-Val-Tyr bond in a synthetic substrate. Does not act on esters of Tyr or Arg =

Synonyms

Acid protease, Aspartate protease, Aspartic proteinase, Aspartic proteinase yscA, EC 3.4.23.6, EC 3.4.23.8, EC 3.4.4.17, Pep4, PEP4 gene product, Pep4p, Pep4p vacuolar proteinase, pepsin-like aspartic proteinase, PRA, preproPrA, proPrA, Protease A, Proteinase A, proteinase A precursor, Proteinase yscA, Proteinase, yeast A, proteinase-A, pseudo-proteinase A, Saccharomyces aspartic proteinase, Saccharomyces cerevisiae aspartic proteinase A, saccharopepsin, vacuolar aspartic proteinase, yeast aspartic proteinase A, Yeast endopeptidase A, yeast proteinase, Yeast proteinase A

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.23 Aspartic endopeptidases

                3.4.23.25 saccharopepsin

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Results	in table
2	Activating Compound
18	AA Sequence
9	Application
1	CAS Registry Number
5	Cloned(Commentary)
6	Crystallization (Commentary)
3	Expression
2	General Information
3	General Stability
1	IC50 Value
50	Inhibitors
47	Ki Value [mM]
39	KM Value [mM]
21	Localization
18	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
76	Organism
12	PDB ID
14	pH Optimum
4	pH Range
4	pH Stability
9	Posttranslational Modification
5	Protein Variants
13	Purification (Commentary)
3	Reaction
1	Reaction Type
43	Reference
1	Source Tissue
4	Specific Activity [micromol/min/mg]
2	Storage Stability
115	Substrates and Products (Substrate)
15	Subunits
75	Synonyms
2	Temperature Optimum [°C]
3	Temperature Stability [°C]
40	Turnover Number [1/s]

Natural Substrates Products

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Natural Substrates Products on EC 3.4.23.25 - saccharopepsin

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

lipid transfer protein 1 + H2O

?

2 entries

procarboxypeptidase Y + H2O

propeptide of carboxypeptidase Y + carboxypeptidase Y

2 entries

proproteinase A + H2O

propeptide of proteinase A + proteinase A

2 entries

proproteinase A + H2O

pseudo-proteinaseA + peptide

Saccharomyces cerevisiae

P07267

autoactivation of the enzyme yields a functional protein cleaved after Ser68, autoactivation can occur in rare situations when PrB is unable to activate PrA

683009

Ser68 is the N-terminal amino acid

-

?

proproteinase B + H2O

propeptide of proteinase B + proteinase B

2 entries

Spt7p + H2O

?

Saccharomyces cerevisiae

-

the enzyme is required for cleavage of Spt7p subunit within SAGA in vitro into SLIK-related Spt7p

717794

-

-

?

additional information

?

-

6 entries

lipid transfer protein 1 + H2O

?

Saccharomyces cerevisiae

-

-

754336

-

-

?

lipid transfer protein 1 + H2O

?

Saccharomyces cerevisiae W303-1A

-

-

754336

-

-

?

procarboxypeptidase Y + H2O

propeptide of carboxypeptidase Y + carboxypeptidase Y

Saccharomyces cerevisiae

-

maturation and activation of procarboxypeptidase Y

683085

-

-

?

procarboxypeptidase Y + H2O

propeptide of carboxypeptidase Y + carboxypeptidase Y

Saccharomyces cerevisiae MT8-1

-

maturation and activation of procarboxypeptidase Y

683085

-

-

?

proproteinase A + H2O

propeptide of proteinase A + proteinase A

Saccharomyces cerevisiae

-

autocatalytic activation of proproteinase A

683085

-

-

?

proproteinase A + H2O

propeptide of proteinase A + proteinase A

Saccharomyces cerevisiae MT8-1

-

autocatalytic activation of proproteinase A

683085

-

-

?

proproteinase B + H2O

propeptide of proteinase B + proteinase B

Saccharomyces cerevisiae

-

maturation and activation of proproteinase B

683085

-

-

?

proproteinase B + H2O

propeptide of proteinase B + proteinase B

Saccharomyces cerevisiae MT8-1

-

maturation and activation of proproteinase B

683085

-

-

?

additional information

?

-

Saccharomyces cerevisiae

-

protein degradation under sporulation conditions is about 30% reduced in proteinase A mutant cells, the differentiation process of sporulation is also disturbed leading to a 40% reduced sporulation frequency in mutant cells

30700

-

-

?

additional information

?

-

Saccharomyces cerevisiae

-

involved in intracellular proteolysis, especially under sporulation conditions

651749

-

651749

?

additional information

?

-

Saccharomyces cerevisiae

P07267

essential to the activities of other yeast vacuolar hydrolases, including proteinase B, aminopeptidase I, and carboxypeptidase Y

683009

-

-

?

additional information

?

-

Saccharomyces cerevisiae

-

essential to the activities of other yeast vacuolar hydrolases, including proteinase B, aminopeptidase I, and carboxypeptidase Y

683009

-

-

?

additional information

?

-

Saccharomyces cerevisiae

-

important for protein turnover after oxidative damage, supplies nitrogen under nutritional stress conditions

688855

-

-

?

additional information

?

-

Saccharomyces cerevisiae

-

required for the activation of carboxypeptidase Y and proteinase B

686781

-

-

?