3.4.23.20: Penicillopepsin

This is an abbreviated version, for detailed information about Penicillopepsin, go to the full flat file.

Reaction

Hydrolysis of proteins with broad specificity similar to that of pepsin A, preferring hydrophobic residues at P1 and P1', but also cleaving Gly20-/-Glu in the B chain of insulin. Clots milk, and activates trypsinogen =

Synonyms

Acid protease A, EC 3.4.23.6, EC 3.4.23.7, mold kinase, More, pencillopepsin-JT1, pencillopepsin-JT2, Penicillium citrinum acid proteinase, Penicillium cyclopium acid proteinase, Penicillium expansum acid proteinase, Penicillium janthinellum acid proteinase, Penicillium janthinellum aspartic proteinase, penicillium kinase, Penicillium roqueforti acid proteinase, penicillopepsin-JT1, penicillopepsin-JT2, penicillopepsin-JT3, Peptidase A, Proteinase, Penicillium aspartic, Proteinase, Penicillium caseicolum aspartic, Proteinase, Penicillium citrinum aspartic, Proteinase, Penicillium cyclopium acid, Proteinase, Penicillium duponti aspartic, Proteinase, Penicillium expansum aspartic, Proteinase, Penicillium janthinellum acid, Proteinase, Penicillium roqueforti aspartic

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.20 Penicillopepsin

Turnover Number

Turnover Number on EC 3.4.23.20 - Penicillopepsin

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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.35 - 34
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
28.8 - 47
Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala amide
45
Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala-Ala amide
-
pH 5.5 (pH-optimum for kcat)
0.3 - 40
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
0.09 - 15
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
0.0019 - 0.36
Ac-Ala-Ala-Lys-(NO2)Phe-amide
0.021
Ac-Ala-Lys-(4-nitro)Phe amide
-
pH 4.3 (pH-optimum for kcat)
0.15 - 0.45
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
0.046 - 0.6
Ac-Ala-Lys-(NO2)Phe-Ala-amide
0.003 - 0.0054
Ac-Ala-Lys-(NO2)Phe-amide
0.003
Ac-Ala-Lys-Ala-(NO2)Phe-amide
-
pH 5.0, 25C, mutant T219S
0.01
Ac-Lys 4-nitrophenyl amide
-
pH 5.5
0.026
Ac-Lys-(4-nitro)Phe amide
-
pH 3.8-4.2 (pH-optimum for kcat)
0.12 - 0.43
Ac-Lys-(NO2)Phe-Ala-Ala-amide
0.042 - 0.4
Ac-Lys-(NO2)Phe-Ala-amide
0.0016 - 0.005
Ac-Lys-(NO2)Phe-amide
5
bovine trypsinogen
-
-
-
6.4 - 11.4
Leu-Ser-(4-nitro)Phe-Nle-Ala-Leu methyl ester
37.3
Pro-Thr-Glu-Phe-(4-nitro)Phe-Arg-Leu
-
-
additional information
additional information
-