3.4.23.19: Aspergillopepsin II
This is an abbreviated version!
For detailed information about Aspergillopepsin II, go to the full flat file.
Reaction
preferential cleavage in B chain of insulin: Asn3-/-Gln, Gly13-/-Ala, Tyr26-/-Thr =
Synonyms
acid proteinase, acid proteinase A, AGP, aspergilloglutamic peptidase, Aspergillus niger var. macrosporus aspartic proteinase, Aspergillus proteinase A, EC 3.4.23.6, More, non-pepsin type acid proteinase, Proctase A, Proteinase A
ECTree
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Engineering
Engineering on EC 3.4.23.19 - Aspergillopepsin II
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D123E/E219D
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site-directed mutagenesis, weak activity against acid-denatured hemoglobin
D125N
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
D137N
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
D148N
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
D160N
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
D170N
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
D220N
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
E152Q
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
E189Q
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
E222Q
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
D123N
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
E219A
-
site-directed mutagenesis, proform mutant, inactive mutant
E219Q
-
site-directed mutagenesis, proform mutant, inactive mutant
N213A
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site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme
N65A
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site-directed mutagenesis, proform mutant, the mutant shows increased activity compared to the wild-type enzyme
Q133A
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site-directed mutagenesis, proform mutant, inactive mutant
Q133E
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site-directed mutagenesis, proform mutant, inactive mutant
Q133E/E219Q
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site-directed mutagenesis, proform mutant, inactive mutant
S162A
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site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme
S167A
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site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme
T126A
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site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme
T134A
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site-directed mutagenesis, proform mutant, the mutant shows similar activity compared to the wild-type enzyme
T192A
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site-directed mutagenesis, proform mutant, the mutant shows increased activity compared to the wild-type enzyme
T268A
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site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme
Y151F
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site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme
Y154F
-
site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme
Y291F
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site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme