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3.4.23.19: Aspergillopepsin II

This is an abbreviated version!
For detailed information about Aspergillopepsin II, go to the full flat file.

Word Map on EC 3.4.23.19

Reaction

preferential cleavage in B chain of insulin: Asn3-/-Gln, Gly13-/-Ala, Tyr26-/-Thr =

Synonyms

acid proteinase, acid proteinase A, AGP, aspergilloglutamic peptidase, Aspergillus niger var. macrosporus aspartic proteinase, Aspergillus proteinase A, EC 3.4.23.6, More, non-pepsin type acid proteinase, Proctase A, Proteinase A

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.19 Aspergillopepsin II

Engineering

Engineering on EC 3.4.23.19 - Aspergillopepsin II

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D123A
-
site-directed mutagenesis, activity is almost at background levels
D123E
-
site-directed mutagenesis, activity not detectable, below 0.2%
D123E/E219D
-
site-directed mutagenesis, weak activity against acid-denatured hemoglobin
D123N
-
site-directed mutagenesis
D125N
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
D137N
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
D148N
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
D160N
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
D170N
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
D208N
-
site-directed mutagenesis
D220N
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
D225N
-
site-directed mutagenesis
D254N
-
site-directed mutagenesis
E128Q
-
site-directed mutagenesis
E141Q
-
site-directed mutagenesis
E152Q
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
E177Q
-
site-directed mutagenesis
E189Q
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
E206Q
-
site-directed mutagenesis
E211Q
-
site-directed mutagenesis
E2154A
-
site-directed mutagenesis
E219A
-
site-directed mutagenesis, activity is almost at background levels
E219D
-
site-directed mutagenesis, activity not detectable, below 0.2%
E219Q
-
site-directed mutagenesis
E222Q
-
site-directed mutagenesis, markedly active toward acid-denatured hemoglobin
Q24E
-
site-directed mutagenesis
D123N
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
E219A
-
site-directed mutagenesis, proform mutant, inactive mutant
E219Q
-
site-directed mutagenesis, proform mutant, inactive mutant
N213A
-
site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme
N65A
-
site-directed mutagenesis, proform mutant, the mutant shows increased activity compared to the wild-type enzyme
Q133A
-
site-directed mutagenesis, proform mutant, inactive mutant
Q133E
-
site-directed mutagenesis, proform mutant, inactive mutant
Q133E/E219Q
-
site-directed mutagenesis, proform mutant, inactive mutant
S162A
-
site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme
S167A
-
site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme
T126A
-
site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme
T134A
-
site-directed mutagenesis, proform mutant, the mutant shows similar activity compared to the wild-type enzyme
T192A
-
site-directed mutagenesis, proform mutant, the mutant shows increased activity compared to the wild-type enzyme
T268A
-
site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme
Y151F
-
site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme
Y154F
-
site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme
Y291F
-
site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme