3.4.22.B78: endopepidase PH1704

This is an abbreviated version!
For detailed information about endopepidase PH1704, go to the full flat file.

Reaction

the enzyme prefers substrates with an Arg residue at the P1 site =

Synonyms

PH1704, PH1704 protease, PhpI

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.B78 endopepidase PH1704

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Results	in table
1	AA Sequence
3	Cloned(Commentary)
6	General Information
11	Inhibitors
1	KM Value [mM]
10	Metals/Ions
3	Molecular Weight [Da]
9	Organism
3	pH Optimum
1	pH Range
8	Protein Variants
2	Purification (Commentary)
4	Reaction
5	Reference
1	Specific Activity [micromol/min/mg]
11	Substrates and Products (Substrate)
6	Subunits
11	Synonyms
3	Temperature Optimum [°C]
1	Temperature Range [°C]

Substrates Products

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Substrates Products on EC 3.4.22.B78 - endopepidase PH1704

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

Ala-Ala-Leu-Arg-7-amido-4-methylcoumarin + H2O

Ala-Ala-Leu-Arg + 7-amino-4-methylcoumarin

Pyrococcus horikoshii

O59413

-

730765

-

-

?

Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O

Ala-Ala-Phe + 7-amino-4-methylcoumarin

Pyrococcus horikoshii

O59413

-

730765

-

-

?

Ala-Ala-Phe-Arg-7-amido-4-methylcoumarin + H2O

Ala-Ala-Phe-Arg + 7-amino-4-methylcoumarin

4 entries

Ala-Arg-7-amido-4-methylcoumarin + H2O

Ala-Arg + 7-amino-4-methylcoumarin

Pyrococcus horikoshii

O59413

-

730765

-

-

?

Ala-Phe-7-amido-4-methylcoumarin + H2O

Ala-Phe + 7-amino-4-methylcoumarin

Pyrococcus horikoshii

O59413

-

730765

-

-

?

Gelatin + H2O

?

Pyrococcus horikoshii

O59413

-

730765

-

-

?

Leu-Arg-7-amido-4-methylcoumarin + H2O

Leu-Arg + 7-amino-4-methylcoumarin

Pyrococcus horikoshii

O59413

-

730765

-

-

?

additional information

?

-

Pyrococcus horikoshii

O59413

the enzyme has both aminopeptidase and endopeptidase activities, substrate specificity, overview. Ala-Phe-7-amido-4-methylcoumarin, and Ala-Ala-Phe-7-amido-4-methylcoumarin are poor substrates. Phe-7-amido-4-methylcoumarin, Ala-7-amido-4-methylcoumarin, Val-7-amido-4-methylcoumarin, Asp-7-amido-4-methylcoumarin, and Ser-7-amido-4-methylcoumarin are no substrates

730765

-

-

?

Ala-Ala-Phe-Arg-7-amido-4-methylcoumarin + H2O

Ala-Ala-Phe-Arg + 7-amino-4-methylcoumarin

Pyrococcus horikoshii

O59413

low activity

730765

-

-

?

Ala-Ala-Phe-Arg-7-amido-4-methylcoumarin + H2O

Ala-Ala-Phe-Arg + 7-amino-4-methylcoumarin

Pyrococcus horikoshii

O59413

Glu12 plays an important role in substrate binding

709586

-

-

?

Ala-Ala-Phe-Arg-7-amido-4-methylcoumarin + H2O

Ala-Ala-Phe-Arg + 7-amino-4-methylcoumarin

Pyrococcus horikoshii

O59413

the enzyme is also identified as an aminopeptidase. Activity with the endopeptidase substrate Ala-Ala-Phe-Arg-7-amido-4-methylcoumarin is 10% compared to the activity with the aminopeptidase substrate L-Arg-7-amido-4-methylcoumarin

730765

-

-

?

Ala-Ala-Phe-Arg-7-amido-4-methylcoumarin + H2O

Ala-Ala-Phe-Arg + 7-amino-4-methylcoumarin

Pyrococcus horikoshii DSM 12428

O59413

Glu12 plays an important role in substrate binding

709586

-

-

?