3.4.22.B75: SENP7 peptidase
This is an abbreviated version!
For detailed information about SENP7 peptidase, go to the full flat file.
Word Map on EC 3.4.22.B75
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3.4.22.B75
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senp7s
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hp1
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heterochromatin
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medicine
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deconjugation
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h3k9me3
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pericentric
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desumoylation
- 3.4.22.B75
- senp7s
- hp1
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heterochromatin
- medicine
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deconjugation
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h3k9me3
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pericentric
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desumoylation
Reaction
Protease that deconjugates SUMO2 and SUMO3 from targeted proteins, but not SUMO1. Catalyzes the deconjugation of poly-SUMO2 and poly-SUMO3 chains. Has very low efficiency in processing full-length SUMO proteins to their mature forms. SENP67 prefers the LRGG-/- sequence versus the QTGG-/- sequence. =
Synonyms
SENP7, SENP7 SUMO-protease, sentrin-specific protease 7, sentrin/small ubiquitin-like modifier-specific protease 7, SUMO protease SENP7, SUMO-specific protease 7, SUMO-specific protease SENP7, SUMO/sentrin/Smt3-specific peptidase
ECTree
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Engineering
Engineering on EC 3.4.22.B75 - SENP7 peptidase
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C745K
mutation does not alter deconjugation rates in comparison with wild-type SENP7
F709W
end point deconjugation reactions using di-SUMO2/3 or poly-SUMO2/3 reveales rates equivalent to or slightly greater than wild-type SENP7. Mutation results in a 2fold higher activity when di-SUMO2 deconjugation rates are measured
V713E
mutation elicits 65-fold reduction in deconjugation rate compared to wild-type activity
V713E/C745K
C745K substitution partially rescues defects observed for SENP7-V713E when present as a double point substitution (SENP7-V713E/C745K)
additional information
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enzyme knockout by siRNA in HeLa cells, complementation of knockdown with siRNA-resistant Flag-SENP7
additional information
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Depletion of SENP7 from NIH-3T3 cells by transfecting a plasmid encoding both a microRNA to downregulate SENP7 (miSENP7) and a GFP mRNA to enable identification of the transfected cells
additional information
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knockdown strategy to deplete SENP7 from NIH-3T3 cells by transfecting a plasmid encoding both a microRNA to downregulate SENP7 and GFP mRNA to enable the identification of microRNA-expressing cells