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3.4.22.B71: SENP2 peptidase

This is an abbreviated version!
For detailed information about SENP2 peptidase, go to the full flat file.

Word Map on EC 3.4.22.B71

Reaction

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Synonyms

(SUMO-1)-specific protease 2, SENP2, sentrin/SUMO-specific protease 2, small ubiquitin-like modifier-specific protease, small ubiquitin-like modifier-specific protease 2, small ubiquitin-related modifier-specific isopeptidase, SUMO-specific isopeptidase, SUMO-specific protease, SUMO-specific protease 2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B71 SENP2 peptidase

Engineering

Engineering on EC 3.4.22.B71 - SENP2 peptidase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C548S
site-directed mutagenesis, active site mutant
C549A
-
catalytically inactive mutant
F393G/K394G
construction of a chimeric enzyme SENP2 mutant with the insertion of loop1 from SENP6 into its sequence causing mutation of F393G/K394G, loop1 insertion is a determinant for SUMO2/3 activity and specificity, structure of SENP2-loop1 in complex with SUMO2, overview. The mutant reveals the details of an interface exclusive to SENP6/7 and the formation of unique contacts between both proteins, and the mutant shows an increase of the proteolytic activity for diSUMO2 and polySUMO2 substrates. The chimeric insertion mutant used for crystallization studies is also mutated at active site residue, C548S, for a stable complex formation
G545D
slight decrease in SUMO-1 processing activity and SUMO-2 processing activity. Decrease in cleavage of the RanGAP1-SUMO-1 conjugate
G545F
decrease in SUMO-1 processing activitya and SUMO-2 processing activity. Increase in SUMO-3 procesing activity
G545S
decrease in SUMO-1 processing activity, SUMO-2 processing activity and SUMO-3 procesing activity. Decrease in cleavage of the RanGAP1-SUMO-1 conjugate
M497A
increase in SUMO-1 processing activity and SUMO-3 procesing activity. Decrease in cleavage of the RanGAP1-SUMO-1 conjugate. Slight decrease in cleavage of the RanGAP1-SUMO-2/3 conjugate
M497L
decrease in SUMO-1 processing activity, SUMO-2 processing activity and SUMO-3 procesing activity
M497N
slight increase in SUMO-1 processing activity, decrease in SUMO-2 processing activity. Decrease in cleavage of the RanGAP1-SUMO-1 conjugate
M497Q
decrease in SUMO-1 processing activity, SUMO-2 processing activity and SUMO-3 procesing activity. Decrease in cleavage of the RanGAP1-SUMO-1 conjugate. Decrease in cleavage of the RanGAP1-SUMO-2/3 conjugate
R576L/K577M
V477A
substitution results in mild effects on processing or deconjugation
C548S
catalytically inactive mutant
additional information